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Advisor(s)
Abstract(s)
The knowledge of the optical properties of biological tissues in a wide spectral range
is highly important for the development of noninvasive diagnostic or treatment procedures. The
absorption coefficient is one of those properties, from which various information about tissue
components can be retrieved. Using transmittance and reflectance spectral measurements acquired
from ex vivo rabbit brain cortex samples allowed to calculate its optical properties in the ultraviolet
to the near infrared spectral range. Melanin and lipofuscin, the two pigments that are related to the
aging of tissues and cells were identified in the cortex absorption. By subtracting the absorption of
these pigments from the absorption of the brain cortex, it was possible to evaluate the true ratios for
the DNA/RNA and hemoglobin bands in the cortex—12.33-fold (at 260 nm), 12.02-fold (at 411 nm)
and 4.47-fold (at 555 nm). Since melanin and lipofuscin accumulation increases with the aging of the
brain tissues and are related to the degeneration of neurons and their death, further studies should
be performed to evaluate the evolution of pigment accumulation in the brain, so that new optical
methods can be developed to aid in the diagnosis and monitoring of brain diseases.
Description
Keywords
Tissue spectroscopy Tissue optical properties Scattering coefficient Absorption coefficient DNA content Blood content Pigment detection
Citation
Gonçalves, T.M., Martins, I.S., Silva, H.F., Tuchin, V.V., Oliveira, L.M. Spectral optical properties of rabbit brain cortex between 200 and 1000 nm. Photochem, vol. 1, no. 2, 190-208.
Publisher
MDPI