Molecular Imprinting of Complex Matrices at Localized Surface Plasmon Resonance Biosensors for Screening of Global Interactions of Polyphenols and Proteins

Guerreiro, Joana Rafaela Lara; Bochenkov, Vladimir E.; Runager, Kasper; Aslan, Hüsnü; Dong, Mingdong; Enghild, Jan J.; Freitas, Victor de; Sales, M. Goreti F.; Sutherland, Duncan S.

http://hdl.handle.net/10400.22/10040

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
ART_GoretiSales_BioMark_2016.pdf		3.69 MB	Adobe PDF	Download

Send Feedback

Authors

Guerreiro, Joana Rafaela Lara

Bochenkov, Vladimir E.

Sutherland, Duncan S.

Abstract(s)

Molecular imprinting polymers (MIP) have been applied to capture and stabilize complex protein matrices at plasmonic sensor surfaces. Ultrathin MIP layers at the surface of gold nanodisks enable the label free quantification of global interactions of polyphenols with protein mixtures. Separate polyphenols (catechin, procyanidin B3- catechin dimer, and PGG-pentagalloyl glucose) give specific and different binding levels to the MIP supported saliva plasmonic sensor. The demonstrated biosensor has application to study bioavailability of polyphenols or evaluation of local retention of small drug molecules.

Keywords

Localized surface plasmon resonance (LSPR) Molecular imprinting Complex protein matrix Polyphenols Biosensing Nanodisks

URI

http://hdl.handle.net/10400.22/10040

Publisher

American Chemical Society

DOI

10.1021/acssensors.5b00054

Collections

ISEP – BioMark – Artigos

Altmetrics

Full item page