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The influence of adipocyte secretome on selected metabolic fingerprints of breast cancer cell lines representing the four major breast cancer subtypes

2023-08-22Journal article Open access
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dc.contributor.authorLuís, Carla
dc.contributor.authorGuerra-Carvalho, Bárbara
dc.contributor.authorBraga, Patrícia C.
dc.contributor.authorGuedes, Carla
dc.contributor.authorPatrício, Emília
dc.contributor.authorAlves, Marco G.
dc.contributor.authorFernandes, Ruben
dc.contributor.authorSoares, Raquel
dc.date.accessioned2024-07-08T08:43:41Z
dc.date.available2024-07-08T08:43:41Z
dc.date.issued2023-08-22
dc.description.abstractMolecular subtype (MS) is one of the most used classifications of breast cancer (BC). Four MSs are widely accepted according to receptor expression of estrogen, progesterone, and HER2. The impact of adipose tissue on BC MS metabolic impairment is still unclear. The present work aims to elucidate the metabolic alterations in breast cancer cell lines representing different MSs subjected to adipocyte associated factors. Preadipocytes isolated from human subcutaneous adipose tissue were differentiated into mature adipocytes. MS representative cell lines were exposed to mature adipocyte secretome. Extracellular medium was collected for metabolomics and RNA was extracted to evaluate enzymatic expression by RT-PCR. Adipocyte secretome exposure resulted in a decrease in the Warburg effect rate and an increase in cholesterol release. HER2+ cell lines (BT-474 and SK-BR-3) exhibited a similar metabolic pattern, in contrast to luminal A (MCF-7) and triple negative (TN) (MDA-MB-231), both presenting identical metabolisms. Anaplerosis was found in luminal A and TN representative cells, whereas cataplerotic reactions were likely to occur in HER2+ cell lines. Our results indicate that adipocyte secretome affects the central metabolism distinctly in each BC MS representative cell line.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLuís, C., Guerra-Carvalho, B., Braga, P. C., Guedes, C., Patrício, E., Alves, M. G., Fernandes, R., & Soares, R. (2023). The Influence of Adipocyte Secretome on Selected Metabolic Fingerprints of Breast Cancer Cell Lines Representing the Four Major Breast Cancer Subtypes. Cells, 12(17), Artigo 17. https://doi.org/10.3390/cells12172123pt_PT
dc.identifier.doi10.3390/cells12172123pt_PT
dc.identifier.eissn2073-4409
dc.identifier.urihttp://hdl.handle.net/10400.22/25739
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationThis research was supported by the FCT—Fundação para a Ciência e Tecnologia by a scholarship granted to Carla Luís (SFRH/BD/146489/2019), Bárbara Guerra-Carvalho (2022.10533.BD), Patrícia C. Braga (UI/BD/150750/2020), Marco G. Alves (2021.03439.CEECIND), UMIB (UIDB/00215/2020 and UIDP/00215/2020), ITR (LA/P/0064/2020) and LAQVREQUIMTE (UIDB/50006/2020), co-funded by FEDER funds through the COMPETE/QREN, FSE/POPH, and POCI—COMPETE 2020 (POCI-01-0145-FEDER-007491) funds and by a research grant of Liga Portuguesa Contra o Cancro (LPCC) and AUSONIA to Carla Luís.pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2073-4409/12/17/2123pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBreast cancerpt_PT
dc.subjectObesitypt_PT
dc.subjectMolecular subtypept_PT
dc.subjectCancer cell metabolismpt_PT
dc.subjectWarburg effectpt_PT
dc.subjectCell culturept_PT
dc.titleThe influence of adipocyte secretome on selected metabolic fingerprints of breast cancer cell lines representing the four major breast cancer subtypespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleCellspt_PT
oaire.citation.volume12(17)pt_PT
person.familyNameGuedes
person.givenNameCarla
person.identifier1888845
person.identifier.ciencia-id2B11-8C00-A60D
person.identifier.orcid0000-0002-3277-7821
person.identifier.ridD-3704-2017
person.identifier.scopus-author-id57447314300
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa1736eb9-65d6-4da5-873e-1b36f7b6e0fb
relation.isAuthorOfPublication.latestForDiscoverya1736eb9-65d6-4da5-873e-1b36f7b6e0fb

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