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Rapid detection of efflux pumps and their relation with drug resistance in yeast cells

2000Journal article Open access
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dc.contributor.authorPrudĂȘncio, Cristina
dc.contributor.authorSansonetty, Filipe
dc.contributor.authorSousa, Maria João
dc.contributor.authorCĂŽrte-Real, Manuela
dc.contributor.authorLeão, Cecı́lia
dc.date.accessioned2023-10-25T13:20:18Z
dc.date.available2023-10-25T13:20:18Z
dc.date.issued2000
dc.description.abstractCell drug resistance can be due to the presence of active efflux pumps (AEP). Identification of yeast cells with a resistance phenotype is important either from a clinical, agricultural or biotechnological point of view. Rapid and reliable methods to detect AEP can be therefore very useful. Some yeast cells change their staining by cal-cein-AM, BCECF-AM, rhodamine 123 and DiOC5,when pretreated with verapamil, CCCP or ATP depletion, or when pretreated with specific antimicrobial agents. This fact may be interpreted as an indication of the presence/absence of AEP. Six yeast species were tested with a flowcytometric method (FCM) and an epifluorescence micro-scopic method (EFM), and ten other species were evalu-ated only by EFM. The minimum inhibitory concentration(MIC) of penconazol, benomyl and cycloheximide for Saccharomyces cerevisiae and Kluyveromyces marxia-nus, were determined by growth inhibition on solid me-dium and were compared to the staining changes de-tected by FCM. The FCM and the EFM allowed the detection of AEP in all the yeast species tested. High MIC values for adrug were related with the presence of at least one AEP indicated by the cytometric data. The FCM revealed to be a robust assay where as the EFM can be used as a preliminary test. It is possible to identify resistance/sensitivity patterns in yeast cells through cytometric detection methods of different efflux pumping systems.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPrudĂȘncio, C., Sansonetty, F., Sousa, M. J., CĂŽrte-Real, M., & LeĂŁo, C. (2000). Rapid detection of efflux pumps and their relation with drug resistance in yeast cells. Cytometry, 39(1), 26–35. https://doi.org/10.1002/(SICI)1097-0320(20000101)39:1<26::AID-CYTO5>3.0.CO;2-Cpt_PT
dc.identifier.doi10.1002/(SICI)1097-0320(20000101)39:1<26::AID-CYTO5>3.0.CO;2-Copen_in_newISSN0pt_PT
dc.identifier.eissn1097-0320
dc.identifier.issn0196-4763
dc.identifier.urihttp://hdl.handle.net/10400.22/23781
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWilleypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/%28SICI%291097-0320%2820000101%2939%3A1%3C26%3A%3AAID-CYTO5%3E3.0.CO%3B2-Cpt_PT
dc.subjectActive efflux pumpspt_PT
dc.subjectYeast drug resistancept_PT
dc.subjectAntimicrobial agentspt_PT
dc.titleRapid detection of efflux pumps and their relation with drug resistance in yeast cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage35pt_PT
oaire.citation.startPage26pt_PT
oaire.citation.titleCytometrypt_PT
oaire.citation.volume39pt_PT
person.familyNamePrudĂȘncio
person.givenNameCristina
person.identifier1200571
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id6508057930
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery881a8ad5-ab13-4e49-89f4-08ca61cc81e3

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