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Development of a biosensor for phosphorylated Tau 181 protein detection in Early-Stage Alzheimer’s disease

2022Journal article Restricted access
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dc.contributor.authorSchneider, Maria
dc.contributor.authorGuillade, Lucía
dc.contributor.authorCorrea-Duarte, Miguel A.
dc.contributor.authorMoreira, Felismina
dc.date.accessioned2023-01-25T16:22:30Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractAlzheimer's disease (AD) is the most common form of dementia in the elderly, and there are still no reliable methods for its early detection. Recently, the phosphorylated protein Tau181 (p-Tau181) was identified as a highly specific biomarker for AD. Therefore, in this work, a new strategy for the development of an electrochemical-based immunosensor for the detection of p-Tau181 is described. For this purpose, a carbon screen-printed electrode (C-SPE) was modified with platinum nanoparticles decorated with multi-walled carbon nanotubes (MWCNTs- PAH /Pt) to enable antibody binding. Scanning electron microscopy, transmission electron microscopy, Raman and X-ray photoelectron spectroscopy were used to study the morphology and crystallinity of the nanomaterials. Cyclic voltammetry and square-wave voltammetry were performed to compare the electrochemical properties of these electrodes. Under optimal conditions, the developed immunosensor exhibited a linear range from 8.6 to 1100 pg/mL, and the detection limit was estimated to be 0.24 pg/mL. This device showed excellent reproducibility and stability with remarkable selectivity for p-Tau181 in serum samples. Overall, this device enables minimally invasive clinical evaluation of p-Tau181 level with high sensitivity through simple operation, which makes this device a promising tool for future point-of-care purposes that will contribute to the technological development of clinical diagnostics.pt_PT
dc.description.sponsorshipAuthors acknowledge funding to POCTEP through the project 2IQBioneuro with the reference (0624_2IQBIONEURO_6_E), entitled, Promotion of an R&I network in biological chemistry for the diagnosis and treatment of neurological diseases, EP-INTERREG V Spain Portugal (POCTEP).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bioelechem.2022.108057pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21867
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation0624_2IQBIONEURO_6_Ept_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1567539422000081pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAlzheimer Biomarkerpt_PT
dc.subjectElectrochemicalpt_PT
dc.subjectImmunosensorpt_PT
dc.titleDevelopment of a biosensor for phosphorylated Tau 181 protein detection in Early-Stage Alzheimer’s diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage108057pt_PT
oaire.citation.titleBioelectrochemistrypt_PT
oaire.citation.volume145pt_PT
person.familyNameSchneider
person.familyNameCorrea-Duarte
person.familyNameMoreira
person.givenNameMaria Eduarda
person.givenNameMiguel A.
person.givenNameFelismina
person.identifier81160
person.identifier1589429
person.identifier.ciencia-idEB1F-576C-4A5D
person.identifier.ciencia-id531D-D97E-5153
person.identifier.ciencia-idC917-6A46-A270
person.identifier.orcid0000-0003-1950-1414
person.identifier.orcid0000-0003-4237-8952
person.identifier.scopus-author-id23488450500
person.identifier.scopus-author-id23486193000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione2e56972-0fcb-4338-a093-222a21380d37
relation.isAuthorOfPublication540d135d-7e45-4f17-a975-0820805046f3
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication.latestForDiscovery540d135d-7e45-4f17-a975-0820805046f3

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