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Adipocyte Secreted Factors Enhance Aggressiveness of Prostate Carcinoma Cells

2015Journal article Open access
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dc.contributor.authorMoreira, Ângela
dc.contributor.authorPereira, Sofia S.
dc.contributor.authorCosta, Madalena
dc.contributor.authorMorais, Tiago
dc.contributor.authorPinto, Ana
dc.contributor.authorFernandes, Rúben
dc.contributor.authorMonteiro, Mariana P.
dc.date.accessioned2019-05-20T14:05:00Z
dc.date.available2019-05-20T14:05:00Z
dc.date.issued2015
dc.description.abstractObesity has been associated with increased incidence and risk of mortality of prostate cancer. One of the proposed mechanisms underlying this risk association is the change in adipokines expression that could promote the development and progression of the prostate tumor cells. The main goal of this study was to evaluate the effect of preadipocyte and adipocyte secretome in the proliferation, migration and invasion of androgen independent prostate carcinoma cells (RM1) and to assess cell proliferation in the presence of the adiposity signals leptin and insulin. RM1 cells were co-cultured in with preadipocytes, adipocytes or cultured in their respective conditioned medium. Cell proliferation was assessed by flow cytometry and XTT viability test. Cell migration was evaluated using a wound healing injury assay of RM1 cells cultured with conditioned media. Cellular invasion of RM1 cells co-cultured with adipocytes and preadipocytes was assessed using matrigel membranes. Preadipocyte conditioned medium was associated with a small increase in RM1 proliferation, while adipocytes conditioned media significantly increased RM1 cell proliferation (p<0.01). Adipocytes also significantly increased the RM1 cells proliferation in co-culture (p <0.01). Cell migration was higher in RM1 cells cultured with preadipocyte and adipocyte conditioned medium. RM1 cell invasion was significantly increased after co-culture with preadipocytes and adipocytes (p <0.05). Insulin also increased significantly the cell proliferation in contrast to leptin, which showed no effect. In conclusion, prostate carcinoma cells seem to be influenced by factors secreted by adipocytes that are able to increase their ability to proliferate, migrate and invade.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1371/journal.pone.0123217pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/13751
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123217pt_PT
dc.subject3T3-L1 Cellspt_PT
dc.subjectAdipocytespt_PT
dc.subjectAnimalspt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectCell Movementpt_PT
dc.subjectCell Proliferationpt_PT
dc.subjectCulture Media, Conditionedpt_PT
dc.subjectFlow Cytometrypt_PT
dc.subjectInsulinpt_PT
dc.subjectLeptinpt_PT
dc.subjectMalept_PT
dc.subjectMicept_PT
dc.subjectProstatic Neoplasmspt_PT
dc.titleAdipocyte Secreted Factors Enhance Aggressiveness of Prostate Carcinoma Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue4pt_PT
oaire.citation.startPagee0123217pt_PT
oaire.citation.titlePLoS ONEpt_PT
oaire.citation.volume10pt_PT
person.familyNameFernandes
person.givenNameRúben
person.identifier635792
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0001-8933-3984
person.identifier.scopus-author-id57640135700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication.latestForDiscovery9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4

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