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Paper-based aptasensor for colorimetric detection of osteopontin

2022Journal article Restricted access
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dc.contributor.authorSantos Pereira, Ana Cláudia
dc.contributor.authorMoreira, Felismina
dc.contributor.authorRodrigues, Lígia
dc.contributor.authorFerreira Sales, Maria Goreti
dc.date.accessioned2023-01-25T15:54:00Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractThis work presents a novel cellulose-based aptasensor for the colorimetric detection of a cancer biomarker, osteopontin (OPN), in point-of-care (PoC) analysis. For this purpose, the cellulose paper was chemically modified with (mercaptopropyl)methyldimetoxisilane to attach the thiolated aptamer, which acts as a biological detection layer. The surface modification was checked by Fourier transform infrared spectroscopy and thermogravimetric analysis. Colorimetric detection was performed using a conventional staining solution, Bradford reagent. The color analysis was performed by evaluating the RGB coordinates provided by the ImageJ program from the photographs taken with a smartphone. Overall, the biosensor shows good sensitivity with a wide linear range (R > 0.998) of 5–1000 ng/mL and a detection limit lower than 5 ng/mL in buffer and commercial human serum solution, after 30 min of incubation. In addition, this aptasensor shows good selectivity to some interfering species such as bovine serum albumin and recombinant OPN. Analytical data obtained from spiked serum samples confirm the accuracy of the method. Importantly, it is a broad-spectrum method that tends to meet the criteria of REASSURED (real-time connectivity, ease of sampling, affordability, specificity, ease of use, speed and robustness, device freedom, and deliverability) for global testing.pt_PT
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Ana Claudia Pereira is a recipient of a fellowship supported by a doctoral advanced training (call NORTE-69-2015-15) funded by the European Social Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aca.2022.339557pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21865
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0145-FEDER-000004pt_PT
dc.relationNORTE-69-2015-15pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0003267022001283?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPaper-based aptasensorpt_PT
dc.subjectColorimetricpt_PT
dc.subjectOsteopontinpt_PT
dc.subjectBradford reagentpt_PT
dc.subjectColor coordinatespt_PT
dc.titlePaper-based aptasensor for colorimetric detection of osteopontinpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage339557pt_PT
oaire.citation.titleAnalytica Chimica Actapt_PT
oaire.citation.volume1198pt_PT
person.familyNameSantos Pereira
person.familyNameMoreira
person.familyNameRodrigues
person.familyNameFerreira Sales
person.givenNameAna Cláudia
person.givenNameFelismina
person.givenNameLigia
person.givenNameMaria Goreti
person.identifier1589429
person.identifier826970
person.identifier.ciencia-id8A10-2A44-A62C
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-id8719-B449-CB82
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0003-1727-8255
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9265-0630
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridC-3151-2009
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id55938875200
person.identifier.scopus-author-id7005174717
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery9c13153d-a73a-49ff-b7f6-c9733bc6725e

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