Publication
Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers
| datacite.subject.fos | Engenharia e Tecnologia | |
| datacite.subject.sdg | 09:Indústria, Inovação e Infraestruturas | |
| dc.contributor.author | Arjum, Ajith Mohan | |
| dc.contributor.author | Deshpande, Sudhaunsh | |
| dc.contributor.author | Dunlop, Tom | |
| dc.contributor.author | Norman, Beth | |
| dc.contributor.author | Oliviera, Daniela | |
| dc.contributor.author | Vulpe, Georgeta | |
| dc.contributor.author | Moreira, Felismina | |
| dc.contributor.author | Sharma, Sanjiv | |
| dc.date.accessioned | 2025-02-07T16:18:33Z | |
| dc.date.available | 2025-02-07T16:18:33Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Phosphorylated Tau proteins are promising biomarkers for the diagnosis and prognosis of Alzheimer's disease. This study presents a novel voltametric sensor using a vanadium MXene polydopamine (VxPDA) redox active composite and a Tau-441-specific polyaniline molecularly imprinted polymer (PANI MIP) for the sensitive detection of Tau-441 in interstitial fluid (ISF) and plasma. The VxPDA/PANI MIP sensor demonstrates a broad detection range of 5 fg/mL to 5 ng/mL (122 aM/L to 122 pM/L) in ISF without the use of redox mediators, with a lower limit of detection (LOD) of 2.3 fg/mL (60 aM/L). Furthermore, a handheld device utilizing this technology successfully detects Tau-441 in artificial serum with high sensitivity (5 fg/mL to 150 fg/mL (122 aM/L to 366 aM/L)) and specificity within a clinically relevant range. The rapid detection time (∼32 min) and low cost (∼£20/device) of this sensor highlight its potential for minimally invasive, early AD diagnosis in clinical settings. This advancement aims to facilitate a transition away from invasive cerebrospinal fluid (CSF)-based diagnostic techniques for AD. | eng |
| dc.description.sponsorship | The authors thank the MRC-AMED UK-Japan project (Multi-analyte prognostic and diagnostic screening in blood and skin for Alzheimer's disease, MR/X02153X/1) for their support. We also thank AIM providing SEM, EDS, and XPS facilities. AIM is funded in part by tzhe EPSRC (EP/M028267/1), the European Regional Development Fund through the Welsh Government (80708) and the Welsh Government's Ser Cymru Program. | |
| dc.identifier.citation | Ajith Mohan Arjun, Sudhaunsh Deshpande, Tom Dunlop, Beth Norman, Daniela Oliviera, Georgeta Vulpe, Felismina Moreira, Sanjiv Sharma, Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers, Biosensors and Bioelectronics: X, Volume 20, 2024, 100513, ISSN 2590-1370, https://doi.org/10.1016/j.biosx.2024.100513. | |
| dc.identifier.doi | 10.1016/j.biosx.2024.100513 | |
| dc.identifier.issn | 2590-1370 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/29432 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S2590137024000773 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.title | Alzheimer's diagnosis beyond cerebrospinal fluid: Probe-Free Detection of Tau Proteins using MXene based redox systems and molecularly imprinted polymers | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Biosensors and Bioelectronics: X | |
| oaire.citation.volume | 20 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |