Repository logo
 
Publication

Toxicity Induced by a Metal Mixture (Cd, Pb and Zn) in the Yeast Pichia kudriavzevii: The Role of Oxidative Stress

2016Journal article Restricted access
Simple item page
dc.contributor.authorMesquita, Vanessa A.
dc.contributor.authorSilva, Cristina F.
dc.contributor.authorSoares, Eduardo
dc.date.accessioned2017-01-09T15:08:16Z
dc.date.embargo2116
dc.date.issued2016
dc.description.abstractThe present work aims to contribute for the elucidation of the role of oxidative stress in the toxicity associated with the exposure of Pichia kudriavzevii to multi-metals (Cd, Pb and Zn). Cells of the non-conventional yeast P. kudriavzevii exposed for 6 h to the action of multi-metals accumulated intracellular reactive oxygen species (ROS), evaluated through the oxidation of the probe 2',7'-dichlorodihydrofluorescein diacetate. A progressive loss of membrane integrity (monitored using propidium iodide) was observed in multi-metal-treated cells. The triggering of intracellular ROS accumulation preceded the loss of membrane integrity. These results suggest that the disruption of membrane integrity can be attributed to the oxidative stress. The exposure of yeast cells to single metal showed that, under the concentrations tested, Pb was the metal responsible for the induction of the oxidative stress. Yeast cells coexposed to an antioxidant (ascorbic acid) and multi-metals did not accumulate intracellular ROS, but loss proliferation capacity. Together, the data obtained indicated that intracellular ROS accumulation contributed to metal toxicity, namely for the disruption of membrane integrity of the yeast P. kudriavzevii. It was proposed that Pb toxicity (the metal responsible for the toxic symptoms under the conditions tested) result from the combination of an ionic mechanism and the intracellular ROS accumulation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00284-016-0987-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/9168
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relation.ispartofseriesCurrent Microbiology;Vol. 72, Issue 5
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00284-016-0987-ypt_PT
dc.subjectCadmiumpt_PT
dc.subjectCell Membranept_PT
dc.subjectLeadpt_PT
dc.subjectOxidative Stresspt_PT
dc.subjectPichiapt_PT
dc.subjectReactive Oxygen Speciespt_PT
dc.subjectZincpt_PT
dc.titleToxicity Induced by a Metal Mixture (Cd, Pb and Zn) in the Yeast Pichia kudriavzevii: The Role of Oxidative Stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage550pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage545pt_PT
oaire.citation.titleCurrent Microbiologypt_PT
oaire.citation.volume72pt_PT
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART3_EVS_CIETI_2016.pdf
Size:
948.3 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – CIETI – Artigos