| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.23 MB | Adobe PDF |
Advisor(s)
Abstract(s)
The development of fast and reliable methods for protein determination are of great relevance to a
diversity of areas from industry to diagnostics. Molecular Imprinted Materials (MIM) has proved to be an
interesting methodology for protein analysis however further studies of the effect of the experimental
parameters and starting materials in the performance of the MIM are still required. Caffeic acid (CAF) is
employed for the
first time as a monomer to tailor a synthetic receptor for a protein target. This was done
by bulk-electropolymerization, applying a constant potential of +2.0 V, for 30 s, on a carbon screen-
printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. Annexin A3
(ANXA3) was selected as protein target due to the fact that this is an emerging biomarker in prostate
cancer.
The assembly of the protein imprinted material (PIM) was followed by Electrochemical Impedance
Spectroscopy (EIS) and Raman Spectroscopy. A non-imprinted material (NIM) was prepared in parallel as
control.
Square wave voltammetry (SWV) was used to monitor the electrochemical signal of the [Fe(CN)6]3 /[Fe
(CN)6]4 redox for the quantification of ANXA3.
The optimized PIM-based device showed average detection limits (LOD) of 0.095 ng/mL, a linear
behavior against log (concentration) between 0.10, and 200 ng/mL and good selectivity. The NIM-based
device showed random behavior against protein concentration. Finally, the PIM-sensor was successfully
applied to the analysis of ANXA3 in spiked urine samples.
Description
Keywords
Annexin A3 Caffeic acid Electrochemical biosensor Protein imprinted material Screen-printed electrodes Urine
Citation
Publisher
Elsevier