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Gold electrode modified by self-assembled monolayers of thiols to determine DNA sequences hybridization
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Orientador(es)
Resumo(s)
The process of immobilization of biological molecules is one of the most important steps in
the construction of a biosensor. In the case of DNA, the way it exposes its bases can result in electrochemical
signals to acceptable levels. The use of self-assembled monolayer that allows a connection to
the gold thiol group and DNA binding to an aldehydic ligand resulted in the possibility of determining
DNA hybridization. Immobilized single strand of DNA (ssDNA) from calf thymus pre-formed from
alkanethiol film was formed by incubating a solution of 2-aminoethanothiol (Cys) followed by glutaraldehyde
(Glu). Cyclic voltammetry (CV) was used to characterize the self-assembled monolayer on the
gold electrode and, also, to study the immobilization of ssDNA probe and hybridization with the complementary
sequence (target ssDNA). The ssDNA probe presents a well-defined oxidation peak at
+0.158 V. When the hybridization occurs, this peak disappears which confirms the efficacy of the annealing
and the DNA double helix performing without the presence of electroactive indicators. The use of
SAM resulted in a stable immobilization of the ssDNA probe, enabling the hybridization detection without
labels. This study represents a promising approach for molecular biosensor with sensible and reproducible
results.
Descrição
Palavras-chave
Single-stranded DNA Genosensor Self-assembled monolayer Gold functionalized electrode
Contexto Educativo
Citação
Editora
Indian Academy of Sciences. Springer