Repository logo
 
Publication

Copper nanoparticles stabilized with cashew gum: Antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell line

2019-08-01Journal article Open access
Simple item page
dc.contributor.authorAmorim, Adriany
dc.contributor.authorMafud, Ana Carolina
dc.contributor.authorNogueira, Silvania
dc.contributor.authorRamos-Jesus, Joilson
dc.contributor.authorAraújo, Alyne Rodrigues de
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorNeta, Maria Brito
dc.contributor.authorAlves, Michel Muálem Moraes
dc.contributor.authorCarvalho, Fernando Aécio Amorim
dc.contributor.authorRufino Arcanjo, Daniel Dias
dc.contributor.authorBraun, Sacha
dc.contributor.authorLópez, Marta Sánchez-Paniagua
dc.contributor.authorLópez-Ruiz, Beatriz
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMascarenhas, Yvonne
dc.contributor.authorSilva, Durcilene
dc.contributor.authorEaton, Peter
dc.contributor.authorLeite, José Roberto Souza Almeida
dc.date.accessioned2020-06-25T10:59:45Z
dc.date.available2020-06-25T10:59:45Z
dc.date.issued2019-08-01
dc.description.abstractCopper nanoparticles stabilized with cashew (CG-CuNPs) were synthesized by reduction reaction using ascorbic acid and sodium borohydride, using the cashew gum (CG) as a natural polymer stabilizer. Dynamic light scattering, atomic force microscopy, Fourier-transform infrared spectroscopy, UV-Vis spectrophotometry, and x-ray diffraction were used to characterize the nanoparticles (CG-CuNPs), and copper was quantified by electrochemical measurement. The UV-vis spectra of the CG-CuNPs confirmed the formation of nanoparticles by appearance of a surface plasmon band at 580 nm after 24 h of reaction. The Fourier-transform infrared spectrum of CG-CuNPs showed the peak at 1704 cm−1 from cashew gum, confirming the presence of the gum in the nanoparticles. The average size of CG-CuNPs by dynamic light scattering and atomic force microscopy was around 10 nm, indicating small, approximately spherical particles. Antimicrobial assays showed that CG-CuNPs had activity against Staphylococcus aureus ATCC 29213 with a minimal inhibitory concentration of 0.64 mM. The cytotoxicity assay on BALB/c murine macrophages showed lower cytotoxic effects for CG-CuNPs than CuSO4·5H2O. Viability cell assays for CG-CuNPs at (0.250 mM) inhibited by 70% the growth of 4T1 LUC (4T1 mouse mammary tumor cell line) and NIH 3T3 cells (murine fibroblast cells) over a 24-h period. Therefore, CG-CuNPs can be used as an antimicrobial agent with lower cytotoxic effects than the CuSO4·5H2O precursor.pt_PT
dc.description.sponsorshipThe author would like to thank at UCM for performingDPV, USP by X-ray diffraction experiment, REQUIMTE/LAQV for FTIR, UnB and UFPI for the cytotoxicityassays, as well as at UFPI for help with DLS, UV-Vis,AFM, and microbiological experiments. This work was supported by Project 400398/2014-1—Desenvolvimento de Nanopartículas Estabilizadas com Goma de Cajueiro para Aplicações Biotecnologicas, financed by CNPq. AlexandraPlácido is grateful to FCT by her grant SFRH/BD/97995/2013, financed by POPH-QREN-Tipologia 4.1-Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1177/0885328219845964pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16005
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSAGE Publicationspt_PT
dc.relation.publisherversionhttps://journals.sagepub.com/doi/10.1177/0885328219845964pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCopper nanoparticlespt_PT
dc.subjectCashew gumpt_PT
dc.subjectCancerpt_PT
dc.subjectAntimicrobialpt_PT
dc.titleCopper nanoparticles stabilized with cashew gum: Antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell linept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage197pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage188pt_PT
oaire.citation.titleJournal of Biomaterials Applicationspt_PT
oaire.citation.volume34pt_PT
person.familyNameDelerue-Matos
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_GRAQ_DELERUE_2019_9.pdf
Size:
714.03 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEP – GRAQ – Artigos