Advisor(s)
Abstract(s)
Antibodies against gliadin are used to detect celiac disease (CD) in patients. An electrochemical
immunosensor for the voltammetric detection of human anti-gliadin antibodies (AGA) IgA and AGA IgG
in real serum samples is proposed. The transducer surface consists of screen-printed carbon electrodes
modified with a carbon nanotube/gold nanoparticle hybrid system, which provides a very useful surface
for the amplification of the immunological interactions. The immunosensing strategy is based on the
immobilization of gliadin, the antigen for the autoantibodies of interest, onto the nanostructured surface.
The antigen–antibody interaction is recorded using alkaline phosphatase labeled anti-human antibodies
and a mixture of 3-indoxyl phosphate with silver ions (3-IP/Ag+) was used as the substrate. The analytical
signal is based on the anodic redissolution of the enzymatically generated silver by cyclic voltammetry.
The electrochemical behavior of this immunosensor was carefully evaluated assessing aspects as sensitivity,
non-specific binding and matrix effects, and repeatability and reproducibility. The results were
supported with a commercial ELISA test.
Description
Keywords
Gliadin Celiac disease Electrochemical immunosensors Point-of-care analysis Screen-printed electrodes Nanomaterials