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Optimization and validation of organochlorine compounds in adipose tissue by SPE-gas chromatography

2012Journal article Open access
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dc.contributor.authorFernandes, Virgínia C.
dc.contributor.authorPestana, Diogo
dc.contributor.authorMonteiro, Rosário
dc.contributor.authorFaria, Gil
dc.contributor.authorMeireles, Manuela
dc.contributor.authorCorreia-Sá, Luísa
dc.contributor.authorTeixeira, Diana
dc.contributor.authorFaria, Ana
dc.contributor.authorCalhau, Conceição
dc.contributor.authorDomingues, Valentina F.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2013-10-04T11:31:08Z
dc.date.available2013-10-04T11:31:08Z
dc.date.issued2012
dc.description.abstractScientific evidence has shown an association between organochlorine compounds (OCC) exposure and human health hazards. Concerning this, OCC detection in human adipose samples has to be considered a public health priority. This study evaluated the efficacy of various solid-phase extraction (SPE) and cleanup methods for OCC determination in human adipose tissue. Octadecylsilyl endcapped (C18-E), benzenesulfonic acid modified silica cation exchanger (SA), poly (styrene-divinylbenzene (EN) and EN/RP18 SPE sorbents were evaluated. The relative sample cleanup provided by these SPE columns was evaluated using gas chromatography with electron capture detection (GC–ECD). The C18-E columns with strong homogenization were found to provide the most effective cleanup, removing the greatest amount of interfering substance, and simultaneously ensuring good analyte recoveries higher than 70%. Recoveries>70% with standard deviations (SD)<15% were obtained for all compounds under the selected conditions. Method detection limits were in the 0.003–0.009 mg/kg range. The positive samples were confirmed by gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). The highest percentage found of the OCC in real samples corresponded to HCB, o,p′-DDT and methoxychlor, which were detected in 80 and 95% of samples analyzed respectively. Copyright © 2012 John Wiley & Sons, Ltd.por
dc.identifier.doi10.1002/bmc.2723pt_PT
dc.identifier.issn0269-3879
dc.identifier.urihttp://hdl.handle.net/10400.22/2183
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherJohn Wiley & Sons, Inc.por
dc.relation.ispartofseriesBiomedical Chromatography; Vol. 26, Issue 12
dc.relation.publisherversionThe definitive version is available at www3.interscience.wiley.com | http://onlinelibrary.wiley.com/doi/10.1002/bmc.2723/abstract;jsessionid=AFE62E8AFD42501F59ED9A90C9F0F28C.f03t02por
dc.subjectOrganochlorine pesticidespor
dc.subjectTCDDpor
dc.subjectSPEpor
dc.subjectGC-ECDpor
dc.subjectGC-MS/MSpor
dc.subjectAdipose tissuepor
dc.titleOptimization and validation of organochlorine compounds in adipose tissue by SPE-gas chromatographypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1501por
oaire.citation.issueIssue 12
oaire.citation.startPage1494por
oaire.citation.titleBiomedical Chromatography
oaire.citation.volumeVol. 26por
person.familyNameDomingues
person.familyNameDelerue-Matos
person.givenNameValentina Maria Fernandes
person.givenNameCristina
person.identifier.ciencia-id4E16-791D-6664
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-3472-849X
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
rcaap.rightsclosedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationbe653ab6-34ec-4329-972a-eee990a7ec66
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscoverybe653ab6-34ec-4329-972a-eee990a7ec66

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