Publication
Electropolymerized, Molecularly Imprinted Polymer on a Screen-Printed Electrode—A Simple, Fast, and Disposable Voltammetric Sensor for Trazodone
| dc.contributor.author | Seguro, Isabel | |
| dc.contributor.author | Rebelo, Patrícia | |
| dc.contributor.author | Pacheco, João | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2023-01-31T09:46:43Z | |
| dc.date.available | 2023-01-31T09:46:43Z | |
| dc.date.issued | 2022-04-06 | |
| dc.description.abstract | In recent years, analytical chemistry has been facing new challenges, particularly in developing low-cost, green, and easy-to-reproduce methods. In this work, a simple, reproducible, and low-cost electrochemical (voltammetric) molecularly imprinted polymer (MIP) sensor was designed specifically for the detection of trazodone (TZD). Trazodone (TZD) is an antidepressant drug consumed worldwide since the 1970s. By combining electropolymerization (surface imprinting) with screen-printed electrodes (SPCEs), the sensor is easy to prepare, is environmentally friendly (uses small amounts of reagents), and can be used for in situ analysis through integration with small, portable devices. The MIP was obtained using cyclic voltammetry (CV), using 4-aminobenzoic acid (4-ABA) as the functional monomer in the presence of TZF molecules in 0.1 M HCl. Non-imprinted control was also constructed in the absence of TZD. Both polymers were characterized using CV, and TZD detection was performed with DPV using the oxidation of TZD. The polymerization conditions were studied and optimized. Comparing the TZD signal for MIP/SPCE and NIP/SPCE, an imprinting factor of 71 was estimated, indicating successful imprinting of the TZD molecules within the polymeric matrix. The analytical response was linear in the range of 5–80 µM, and an LOD of 1.6 µM was estimated. Selectivity was evaluated by testing the sensor for molecules with a similar structure to TZD, and the ability of MIP/SPCE to selectively bind to TZD was proven. The sensor was applied to spiked tap water samples and human serum with good recoveries and allowed for a fast analysis (around 30 min). | pt_PT |
| dc.description.sponsorship | Project Farmasense (ref. 39957) is sponsored by Sistema de Incentivos à Investigação e Desenvolvimento Tecnológico do Portugal 2020, by Programa Operacional do Norte (NORTE 2020) and Fundo Europeu de Desenvolvimento Regional (FEDER). João Pacheco is grateful to FCT for his postdoc grant (SFRH/BPD/101419/2014), financed by POPH-QREN-Tipologia 4.1-Formação Avançada, funded by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. Isabel Seguro is grateful to the project Farmasense (39957) that was funded by Sistema de Incentivos à Investigação e Desenvolvimento Tecnológico de Portugal 2020, through the Programa Operacional do Norte (NORTE 2020) and the Fundo Europeu de Desenvolvimento Regional (FEDER). Patrícia Rebelo is grateful to FCT (Fundação para a Ciência e Tecnologia) for her PhD grant (SFRH/BD/132384/2017). This work was financially supported by Portuguese national funds through projects UIDB/50006/2020, UIDP/50006/2020, LA/P/0008/2020 and PTDC/QUI-QAN/3899/2021 from the Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior (MCTES). The authors would like to thank the EU and FCT/UEFISCDI/FORMAS for funding, in the frame of the collaborative international consortium REWATER financed under the ERA-NET Cofund WaterWorks 2015 Call. This ERA-NET is an integral part of the 2016 Joint Activities developed by the Water Challenges for a Changing World Joint Programme Initiative (Water JPI). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.3390/s22072819 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/22021 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | LA/P/0008/2020 | pt_PT |
| dc.relation | Construction of point-of-care molecularly imprinted electrochemical devices for cancer biomarkers detection | |
| dc.relation | Computer-aided design of molecular imprinted electrochemical sensors for analysis of pharmaceuticals in waters | |
| dc.relation | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| dc.relation | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| dc.relation.publisherversion | https://www.mdpi.com/1424-8220/22/7/2819 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Trazodone | pt_PT |
| dc.subject | Antidepressant drugs | pt_PT |
| dc.subject | Dlectrochemical sensor | pt_PT |
| dc.subject | Molecularly imprinted polymer | pt_PT |
| dc.subject | Disposable analytical devices | pt_PT |
| dc.title | Electropolymerized, Molecularly Imprinted Polymer on a Screen-Printed Electrode—A Simple, Fast, and Disposable Voltammetric Sensor for Trazodone | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Construction of point-of-care molecularly imprinted electrochemical devices for cancer biomarkers detection | |
| oaire.awardTitle | Computer-aided design of molecular imprinted electrochemical sensors for analysis of pharmaceuticals in waters | |
| oaire.awardTitle | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| oaire.awardTitle | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F101419%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F132384%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-QAN%2F3899%2F2021/PT | |
| oaire.citation.issue | 7 | pt_PT |
| oaire.citation.startPage | 2819 | pt_PT |
| oaire.citation.title | Sensors | pt_PT |
| oaire.citation.volume | 22 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 3599-PPCDT | |
| person.familyName | Mesquita Guimarães Seguro Pereira Soares | |
| person.familyName | Rebelo | |
| person.familyName | Pacheco | |
| person.familyName | Delerue-Matos | |
| person.givenName | Maria Isabel | |
| person.givenName | Patrícia | |
| person.givenName | João | |
| person.givenName | Cristina | |
| person.identifier.ciencia-id | 591C-F638-0BB2 | |
| person.identifier.ciencia-id | 6112-1293-B25E | |
| person.identifier.ciencia-id | 8D13-F178-F44D | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0001-6854-7262 | |
| person.identifier.orcid | 0000-0001-5209-668X | |
| person.identifier.orcid | 0000-0001-8366-0490 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 35519962400 | |
| person.identifier.scopus-author-id | 6603741848 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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