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Flexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarker

2022Journal article Open access
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dc.contributor.authorMartins, Gabriela V.
dc.contributor.authorRiveiro Rodriguez, Antonio
dc.contributor.authorChiussi, Stefano
dc.contributor.authorSales, Goreti
dc.date.accessioned2023-01-25T15:47:46Z
dc.date.available2023-01-25T15:47:46Z
dc.date.issued2022
dc.description.abstractIn recent years, the development of flexible and wearable devices for healthcare and biomedical applications has become an emerging technological goal, particularly with personalized medicine on the rise. As a response to the increasing demand for in-situ sensing platforms that fulfil some essential requirements like sensitivity, reproducibility and high stability, electrochemical sensors have boosted their way for innovative approaches. So, high-quality flexible sensing strategies are still a demand for local monitoring. Herein, a flexible three-electrode system was fabricated on transparent polymeric sheet substrate through physical deposition of gold as working, counter, and reference electrodes. Along the fabrication process, the electrochemical performance of these electrodes was assessed by means of cyclic voltammetry (CV) while gold adherence to the plastic material was continuously improved. Afterwards, a high-performance molecularly-imprinted sensing film inspired by natural recognition mechanism was assembled through electropolymerization of phenol monomer, in the presence of 3-nitrotyrosine (3-NT), directly on the gold surface. Under the optimized conditions, the flexible (bio)sensor platform was able to detect the presence of 3-NT over the concentration range 10 pg/mL – 1 μg/mL, enabling one of the lower limits of detection found in the literature (1.13 pg/mL or 24.9 pM). The obtained (bio)sensor displayed good reproducibility, stability and selectivity over the chosen interfering substances. Overall, the developed electrochemical device may serve as a flexible, miniaturized, and reliable platform, with potential to be applied in the future as wearable sensing technology.pt_PT
dc.description.sponsorshipThe authors acknowledge the financial support from IBEROS project (Instituto de Bioingeniería en Red para el Envejecimiento Saludable, INTERREG POCTEP/0245_IBEROS_1_E) supported by FEDER, also within the cooperation region of Galicia/Spain and North of Portugal, and the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of PEst UIDB/04469/2020 unit.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biosx.2022.100107pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21864
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationINTERREG POCTEP/0245_IBEROS_1_Ept_PT
dc.relationCentre of Biological Engineering of the University of Minho
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2590137022000036?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMolecularly-imprinted polymerpt_PT
dc.subjectFlexible substratept_PT
dc.subjectGold-based electrodespt_PT
dc.subjectElectrochemical (bio)sensorpt_PT
dc.subjectOxidative stress biomarkerpt_PT
dc.titleFlexible sensing devices integrating molecularly-imprinted polymers for the detection of 3-nitrotyrosine biomarkerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Biological Engineering of the University of Minho
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT
oaire.citation.startPage100107pt_PT
oaire.citation.titleBiosensors and Bioelectronics: Xpt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMartins
person.familyNameRiveiro Rodriguez
person.familyNameChiussi
person.familyNameFerreira Sales
person.givenNameGabriela
person.givenNameAntonio
person.givenNameStefano
person.givenNameMaria Goreti
person.identifier114175
person.identifier970285
person.identifier826970
person.identifier.ciencia-id0219-87C9-5E5B
person.identifier.ciencia-id491E-93FD-7001
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0001-6739-1526
person.identifier.orcid0000-0002-5187-9508
person.identifier.orcid0000-0002-3933-8725
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridH-9741-2015
person.identifier.ridD-6218-2014
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id55918092100
person.identifier.scopus-author-id12789998400
person.identifier.scopus-author-id7004516152
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication4f3a59a7-f683-4956-b7ce-0a4b20b174c6
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