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Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen

2019-09-15Journal article Open access
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dc.contributor.authorSilva, Nádia
dc.contributor.authorAlmeida, Cláudio M.R.
dc.contributor.authorMagalhães, Júlia M.C.S.
dc.contributor.authorGonçalves, Maria P.
dc.contributor.authorFreire, Cristina
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2020-07-10T13:16:30Z
dc.date.available2020-07-10T13:16:30Z
dc.date.issued2019-09-15
dc.description.abstractThis work reports a new paper-based sensing platform and its application in a label-free potentiometric immunosensor for Salmonella typhimurium detection based on the blocking surface principle. A paper-based strip electrode was integrated with a filter paper pad which acted as a reservoir of the internal solution. The design offers a convenient platform for antibody immobilization and sampling, proving also that is a simple and affordable methodology to control an ionic flux through a polymer membrane. Two different immunosensing interfaces were assembled on the developed paper-strip electrode. The simplest interface relied on direct conjugation of the antibody to the polymer membrane and the second one resorted to an intermediate layer of a polyamidoamine dendrimer, with an ethylenediamine core from the fourth generation. Electrochemical impedance spectroscopy was used to assess the successive interface modification steps and the resulting analytical performance of both immunosensors was compared. For such, the potential shift derived from the blocking effect of the ionic flux caused by antigen-antibody conjugation was correlated with the logarithm of the Salmonella typhimurium concentration in the sample. In optimized conditions, a limit of detection of 5 cells mL-1 was achieved. As a proof-of-concept, the proposed method was applied to apple juice samples, demonstrating to be a suitable prototype to be used in real scenarios in useful time (<1 h assay).pt_PT
dc.description.sponsorshipThis work received financial support from the European Union and National Funds(FCT, Fundação para a Ciência e Tecnologia) through projects UID/QUI/50006/2019 and Norte-01-0145-FEDER-000011-RL1–QUALIFOOD. N.F.D Silva is grateful to FCT grant SFRH/BD/112414/2015, financed by POPH–QREN–Tipologia 4.1–FormaçãoAvançada,subsidizedbyFSEandMCTES.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bios.2019.111317pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16093
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0956566319303823?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectPaper-basedpt_PT
dc.subjectPotentiometrypt_PT
dc.subjectImmunosensorpt_PT
dc.subjectLabel-freept_PT
dc.subjectFoodborne pathogenspt_PT
dc.titleDevelopment of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage111317pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume141pt_PT
person.familyNameSilva
person.familyNameDelerue-Matos
person.givenNameNádia
person.givenNameCristina
person.identifier.ciencia-id1A1B-4743-7EC5
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-7759-5768
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridA-2400-2016
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id56622286500
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa2838665-cbc7-48cb-954d-9e46c73c3aaa
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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