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HPLC-DAD, ESI-MS/MS, and NMR of Lycopene Isolated From P. guajava L. and Its Biotechnological Applications

2018-03Journal article Open access
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dc.contributor.authorAmorim, Adriany G. N.
dc.contributor.authorSouza, Jessica M. T.
dc.contributor.authorSantos, Raimunda C.
dc.contributor.authorGullón, Beatriz
dc.contributor.authorOliveira, Ana
dc.contributor.authorSantos, Luiz F. A.
dc.contributor.authorVirgino, Adamor L. E.
dc.contributor.authorMafud, Ana C.
dc.contributor.authorPetrilli, Helena M.
dc.contributor.authorMascarenhas, Yvonne P.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorPintado, Manuela E.
dc.contributor.authorLeite, José R. S. A.
dc.date.accessioned2019-10-07T14:15:59Z
dc.date.available2019-10-07T14:15:59Z
dc.date.issued2018-03
dc.description.abstractPsidium guajava L. have been reported to be a rich source of antioxidant compounds. Its carotenoids have been highlighted by their high antioxidant capacity, which offers several benefits for human health. In this sense, lycopene isomers need to be identified. In this work, the comprehensive chemical characterization, by HPLC‐DAD, MS/MS, and NMR, of lycopene isolated from P. guajava L., antioxidant and antimicrobial activity of lycopene extracts and isolated lycopene are evaluated. The FTIR results reported a structure with Z configuration, confirmed by UV‐Vis, with λmax = 448, 473, and 505 nm for 5‐Z lycopene. Furthermore, MS/MS positive ionization shows one fragment m/z 309 [M‐227]+, relatively abundant for isolated lycopene. Experimental and Theoretical NMR studies revealed that guava may contain 5‐Z lycopene because of the similarity found among the peaks. Lycopene extracts presented higher antioxidant activity than isolated lycopene, from both P. guajava L. and tomato, when measured by ABTS and ORAC (r2 = 0.9995 and r2 = 0.9992, respectively). In addition, lycopene extract shows antibacterial efficacy against E. coli, S. aureus, and L. innocua, presenting MBC values of 20 mg mL−1. These results suggest that lycopene extract have potential applicability for food, cosmetics, and pharmaceutical industry.Practical Applications: Lycopene from P. guajava L. is characterized by HPLCDAD, MS, NMR, FTIR, and X-Ray, presented antioxidant capacity by ORAC and antibacterial efficacy against food pathogens.pt_PT
dc.description.sponsorshipA. A. is grateful to CAPES by PDSE process no 99999.004236/2014-09 in the CBQF of the Portuguese Catholic University (UCP), supported by National Funds from FCT FundacS~ao para a Ci^encia e a Tecnologia through the project PEst-OE/EQB/LA0016/2013. BIOTEC is grateful to Phytobios LTDA and to Delta of Parnaíba Herbarium (HDELTA) UFPI. B. G. and A. O. are grateful to Foundation to Science and Technology (FCT) fellowship reference SFRH/BPD/79941/2011 and PhD grant SFRH/BD/75041/2010, respectively. A. M. is grateful to FAPESP (grant 2014/02282-6). Y. M. was supported by CNPq (Grant 165177/2014-4).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/ejlt.201700330
dc.identifier.urihttp://hdl.handle.net/10400.22/14709
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley-VCH Verlagpt_PT
dc.relationPhD/SFRH/BD/75041/2010pt_PT
dc.relation165177/2014‐4pt_PT
dc.relationPDSE/99999.004236/2014‐09pt_PT
dc.relation2014/02282‐6pt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/full/10.1002/ejlt.201700330pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBioactivitypt_PT
dc.subjectESI–MS/MSpt_PT
dc.subjectHPLC‐DADpt_PT
dc.subjectLycopenept_PT
dc.subjectNMRpt_PT
dc.titleHPLC-DAD, ESI-MS/MS, and NMR of Lycopene Isolated From P. guajava L. and Its Biotechnological Applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0016%2F2013/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage1700330pt_PT
oaire.citation.titleEuropean Journal of Lipid Science and Technologypt_PT
oaire.citation.volume120pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameDelerue-Matos
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isProjectOfPublication40282dfb-1771-4687-905c-7cc0975a6a3a
relation.isProjectOfPublication.latestForDiscovery40282dfb-1771-4687-905c-7cc0975a6a3a

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