Repository logo
 
Publication

Practical Quality Control: Comparision of Methods on the Quantification of Stationary Phases in Paper and Thin-Layer Chromatographic Systems

2010-10-09Lecture Open access
Simple item page
dc.contributor.authorCunha, Lídia
dc.contributor.authorFolgado, Vítor
dc.contributor.authorFerreira, Sara
dc.contributor.authorLourenço, Leonel
dc.contributor.authorOsório, Soraia
dc.contributor.authorOliveira, Marta
dc.contributor.authorSousa, Maria
dc.contributor.authorAmorim, Inês
dc.contributor.authorCastro, Rosa
dc.contributor.authorMetello, Luís F.
dc.date.accessioned2015-05-27T14:01:20Z
dc.date.available2015-05-27T14:01:20Z
dc.date.issued2010-10-09
dc.description.abstractIntroduction: Paper and thin layer chromatography methods are frequently used in Classic Nuclear Medicine for the determination of radiochemical purity (RCP) on radiopharmaceutical preparations. An aliquot of the radiopharmaceutical to be tested is spotted at the origin of a chromatographic strip (stationary phase), which in turn is placed in a chromatographic chamber in order to separate and quantify radiochemical species present in the radiopharmaceutical preparation. There are several methods for the RCP measurement, based on the use of equipment as dose calibrators, well scintillation counters, radiochromatografic scanners and gamma cameras. The purpose of this study was to compare these quantification methods for the determination of RCP. Material and Methods: 99mTc-Tetrofosmin and 99mTc-HDP are the radiopharmaceuticals chosen to serve as the basis for this study. For the determination of RCP of 99mTc-Tetrofosmin we used ITLC-SG (2.5 x 10 cm) and 2-butanone (99mTc-tetrofosmin Rf = 0.55, 99mTcO4- Rf = 1.0, other labeled impurities 99mTc-RH RF = 0.0). For the determination of RCP of 99mTc-HDP, Whatman 31ET and acetone was used (99mTc-HDP Rf = 0.0, 99mTcO4- Rf = 1.0, other labeled impurities RF = 0.0). After the development of the solvent front, the strips were allowed to dry and then imaged on the gamma camera (256x256 matrix; zoom 2; LEHR parallel-hole collimator; 5-minute image) and on the radiochromatogram scanner. Then, strips were cut in Rf 0.8 in the case of 99mTc-tetrofosmin and Rf 0.5 in the case of 99mTc-HDP. The resultant pieces were smashed in an assay tube (to minimize the effect of counting geometry) and counted in the dose calibrator and in the well scintillation counter (during 1 minute). The RCP was calculated using the formula: % 99mTc-Complex = [(99mTc-Complex) / (Total amount of 99mTc-labeled species)] x 100. Statistical analysis was done using the test of hypotheses for the difference between means in independent samples. Results:The gamma camera based method demonstrated higher operator-dependency (especially concerning the drawing of the ROIs) and the measures obtained using the dose calibrator are very sensitive to the amount of activity spotted in the chromatographic strip, so the use of a minimum of 3.7 MBq activity is essential to minimize quantification errors. Radiochromatographic scanner and well scintillation counter showed concordant results and demonstrated the higher level of precision. Conclusions: Radiochromatographic scanners and well scintillation counters based methods demonstrate to be the most accurate and less operator-dependant methods.por
dc.identifier.urihttp://hdl.handle.net/10400.22/6126
dc.language.isoengpor
dc.titlePractical Quality Control: Comparision of Methods on the Quantification of Stationary Phases in Paper and Thin-Layer Chromatographic Systemspor
dc.typelecture
dspace.entity.typePublication
oaire.citation.titleVienna 2010 - Annual Congress of the European Association of Nuclear Medicinepor
person.familyNameCunha
person.familyNameAmorim Sousa
person.givenNameLídia
person.givenNameMaria Manuela
person.identifier.ciencia-idCD1D-1B47-A971
person.identifier.orcid0000-0002-9260-8093
person.identifier.orcid0009-0006-1887-4840
rcaap.rightsopenAccesspor
rcaap.typelecturepor
relation.isAuthorOfPublicationed51e8c5-a1c2-40e6-b976-57af77446e1b
relation.isAuthorOfPublication10df22ed-9823-452e-89a8-8cbe89e717ec
relation.isAuthorOfPublication.latestForDiscoveryed51e8c5-a1c2-40e6-b976-57af77446e1b

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Practical Quality Control_Comparision of Methods on the Quantification of Stationary Phases in Paper and Thin-Layer Chromatographic Systems.pdf
Size:
219.04 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESS - MN - Comunicações em eventos científicos