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An all-in-one approach for self-powered sensing: A methanol fuel cell modified with a molecularly imprinted polymer for cancer biomarker detection

2022Journal article Restricted access
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dc.contributor.authorCarneiro, Liliana P.T
dc.contributor.authorPinto, Alexandra M.F.R.
dc.contributor.authorMendes, Adélio
dc.contributor.authorFerreira Sales, Maria Goreti
dc.date.accessioned2023-01-25T15:13:57Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractThis work describes the development of an innovative electrochemical biosensor comprehending a passive direct methanol fuel cell (DMFC) assembly, modified by a layer of a molecularly imprinted polymer (MIP) on a carbon fabric anode electrode containing Pt/Ru nanoparticles. This MIP film was prepared from poly(3,4-ethylenedioxythiophene) (PEDOT) and polypyrrole (PPy) obtained by in situ electropolymerization of the corresponding monomers on the anode electrode surface. This MIP film is designed to detect an important cancer biomarker- carcinoembryonic antigen (CEA). This innovative, all-in-one device works in a simple way. First, CEA is incubated on the anode container of the fuel cell, then methanol is added, followed by the response evaluation (polarization curves determination). As CEA selectively interacts with the MIP film, it blocks the methanol's access to the Pt catalyst, remains specific bonded, and interferes with the subsequent polarization curves of the DMFC. Polarization curves obtained in the presence of standard solutions prepared in buffer and human serum confirmed linear responses of log CEA concentration ranging from 30 to 30 000 ng/mL in both media. The biosensor DMFC showed a sensitive response with a detection limit of 4.41 ng/mL when an aqueous 0.05 M methanol solution was used as fuel. When methanol was replaced by an ethanol solution of the same concentration (using the same setup developed for the DMFC), the lower detection limit of 3.52 ng/mL was obtained. Overall, the obtained results show that methanol/ethanol fuel cells operating without flow-through can be successfully used for the fabrication of self-powered biosensors. The novel biosensor concept presented here is simple, inexpensive, and effective, and can be further developed to meet point-of-care requirements.pt_PT
dc.description.sponsorshipThe authors acknowledge the financial support of EU-Horizon 2020 (Symbiotic, FET-Open, GA665046) and LPTC (Grant reference SFRH/ BD/122954/2016) acknowledge Fundação para a Ciência e Tecnologia for the financial support. POCI (FEDER) also supported this work via CEFT, project UIDP/00532/2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jelechem.2022.116009pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21862
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationGA665046pt_PT
dc.relationSFRH/ BD/122954/2016pt_PT
dc.relationTransport Phenomena Research Center
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1572665722000017?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSelf-powered biosensorpt_PT
dc.subjectPassive direct methanol fuel cellpt_PT
dc.subjectMolecularly imprinted polymer filmpt_PT
dc.subjectCarcinoembryonic antigenpt_PT
dc.subjectHybrid electrochemical biosensorpt_PT
dc.titleAn all-in-one approach for self-powered sensing: A methanol fuel cell modified with a molecularly imprinted polymer for cancer biomarker detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTransport Phenomena Research Center
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00532%2F2020/PT
oaire.citation.startPage116009pt_PT
oaire.citation.titleJournal of Electroanalytical Chemistrypt_PT
oaire.citation.volume906pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameTeixeira Carneiro
person.familyNameFerreira Rodrigues Pinto
person.familyNameMAGALHÃES MENDES
person.familyNameFerreira Sales
person.givenNameLiliana Patrícia
person.givenNameAlexandra
person.givenNameADÉLIO MIGUEL
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-id011B-41E0-E059
person.identifier.ciencia-id7518-FD35-9DFD
person.identifier.ciencia-idA415-EE94-902E
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-2937-5186
person.identifier.orcid0000-0003-2472-3265
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa522b9a5-f365-497b-9d30-d54a426d406e
relation.isAuthorOfPublicationea0c8b9a-8fba-4d53-a9fe-6275e978e55d
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relation.isAuthorOfPublication.latestForDiscoverya522b9a5-f365-497b-9d30-d54a426d406e
relation.isProjectOfPublication12348463-df26-47f0-816c-711367451859
relation.isProjectOfPublication.latestForDiscovery12348463-df26-47f0-816c-711367451859

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