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Third-generation electrochemical biosensor based on nitric oxide reductase immobilized in a multiwalled carbon nanotubes/1-n-butyl-3-methylimidazolium tetrafluoroborate nanocomposite for nitric oxide detection

2019-04-15Journal article Restricted access
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dc.contributor.authorGomes, Filipa O.
dc.contributor.authorMaia, Luísa B.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMoura, Isabel
dc.contributor.authorMoura, José J.G.
dc.contributor.authorMorais, Simone
dc.date.accessioned2019-10-07T13:37:39Z
dc.date.embargo2119
dc.date.issued2019-04-15
dc.description.abstractNitric oxide (NO) has a crucial role in signaling and cellular physiology in humans. Herein, a novel third-generation biosensor based on the Marinobacter hydrocarbonoclasticus metalloenzyme (nitric oxide reductase (NOR)), responsible for the NO reduction in the denitrifying processes, was developed through the direct adsorption of a new nanocomposite (multiwalled carbon nanotubes (MWCNTs)/1-n-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4)/NOR) onto a pyrolytic graphite electrode (PGE) surface. The NOR direct electron transfer behavior (formal potential of -0.255 ± 0.003 V vs. Ag/AgCl) and electrocatalysis towards NO reduction (−0.68 ± 0.03 V vs. Ag/AgCl) of the PGE/[MWCNTs/BMIMBF4/NOR] biosensor were investigated in phosphate buffer at pH 6.0. Large enzyme loading (2.04 × 10−10 mol/cm2), acceptable electron transfer rate between NOR and the PGE surface (ks = 0.35 s-1), and high affinity for NO (Km = 2.17 μmol L-1) were observed with this biosensor composition. A linear response to NO concentration (0.23–4.76 μmol L-1) was perceived with high sensitivity (0.429 μA/μmolL-1), a detection limit of 0.07 μmol L-1, appropriate repeatability (9.1% relative standard deviations (RSD)), reproducibility (6.0–11% RSD) and 80–102% recoveries. The biosensor was stable during 1 month retaining 79–116% of its initial response. These data confirmed that NOR incorporated in the MWCNTs/BMIMBF4 nanocomposite can efficiently maintain its bioactivity paving a new and effective way for NO biosensing.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.snb.2019.01.074
dc.identifier.urihttp://hdl.handle.net/10400.22/14705
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPTDC/ASP-PES/29547/2017 - CECs(Bio)Sensing - (Bio)sensores para avaliação de contaminantes emergentes em produtos da pescapt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0925400519301029?via%3Dihubpt_PT
dc.subjectDirect electron transferpt_PT
dc.subjectEnzymatic biosensorpt_PT
dc.subjectNitric oxide reductasept_PT
dc.subjectNitric oxidept_PT
dc.subjectMultiwalled carbon nanotubespt_PT
dc.subjectRoom temperature ionic liquidpt_PT
dc.titleThird-generation electrochemical biosensor based on nitric oxide reductase immobilized in a multiwalled carbon nanotubes/1-n-butyl-3-methylimidazolium tetrafluoroborate nanocomposite for nitric oxide detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage452pt_PT
oaire.citation.startPage445pt_PT
oaire.citation.titleSensors and Actuators B: Chemicalpt_PT
oaire.citation.volume285pt_PT
person.familyNameDelerue-Matos
person.familyNameMorais
person.givenNameCristina
person.givenNameSimone
person.identifier1598822
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0001-6433-5801
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
person.identifier.scopus-author-id7007053747
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication.latestForDiscovery7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9

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