Repository logo
 
Publication

Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria

2022-05-16Journal article Restricted access
Simple item page
dc.contributor.authorSilva, Sandra G.
dc.contributor.authorPinheiro, Marina
dc.contributor.authorPereira, Rui
dc.contributor.authorDias, Ana Rita
dc.contributor.authorFerraz, Ricardo
dc.contributor.authorPrudêncio, Cristina
dc.contributor.authorEaton, Peter J.
dc.contributor.authorReis, Salette
dc.contributor.authorVale, M. Luísa C. do
dc.date.accessioned2022-06-02T15:03:51Z
dc.date.available2022-06-02T15:03:51Z
dc.date.issued2022-05-16
dc.description.abstractThe antimicrobial activity of two serine derived gemini cationic surfactants, amide (12Ser)2CON12 and ester (12Ser)2COO12, was tested using sensitive, E. coli ATCC 25922 and S. aureus ATCC 6538, and resistant, E. coli CTX M2, E. coli TEM CTX M9 and S. aureus ATCC 6538 and S. aureus MRSA ATCC 43300 Gram-positive and Gram-negative bacteria strains. Very low MIC values (5 μM) were found for the two resistant strains E.coli TEM CTX M9 and S. aureus MRSA ATCC 43300, in the case of the amide derivative, and for S. aureus MRSA ATCC 43300, in the case of the ester derivative. The interaction of the serine amphiphiles with lipid-model membranes (DPPG and DPPC) was investigated using Langmuir monolayers. A more pronounced effect on the DPPG than on the DPPC monolayer was observed. The effect induced by the surfactants on bacteria membrane was explored by Atomic Force Microscopy. A clear disruption of the bacteria membrane was observed for E. coli TEM CTX M9 upon treatment with (12ser)2CON12, whereas for the S. aureus MRSA few observable changes in cell morphology were found after treatment with either of the two surfactants. The cytotoxicity of the two compounds was assessed by hemolysis assay on human red blood cells (RBC). The compounds were shown to be non-cytotoxic up to 10 μM. Overall, the results reveal a promising potential, in particular of the amide derivative, as antimicrobial agent for two strains of antibiotic resistant bacteria.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, S. G., Pinheiro, M., Pereira, R., Dias, A. R., Ferraz, R., Prudêncio, C., Eaton, P. J., Reis, S., & do Vale, M. L. C. (2022). Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1864(9), 183969. https://doi.org/https://doi.org/10.1016/j.bbamem.2022.183969pt_PT
dc.identifier.doi10.1016/j.bbamem.2022.183969pt_PT
dc.identifier.eissn1879-2642
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/10400.22/20583
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0005273622001079?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectGemini surfactantspt_PT
dc.subjectSerinept_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectLangmuir monolayerspt_PT
dc.subjectHemolytic activitypt_PT
dc.titleSerine-based surfactants as effective antimicrobial agents against multiresistant bacteriapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleBiochimica et Biophysica Acta (BBA) - Biomembranespt_PT
oaire.citation.volume1864pt_PT
person.familyNameda Silva Dias
person.familyNameFerraz
person.familyNamePrudêncio
person.givenNameAna Rita
person.givenNameRicardo
person.givenNameCristina
person.identifier1200571
person.identifier.ciencia-idA315-FD53-E1DC
person.identifier.ciencia-id001E-71CE-F92D
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0003-4006-1185
person.identifier.orcid0000-0002-1761-117X
person.identifier.orcid0000-0002-9920-936X
person.identifier.ridG-5639-2011
person.identifier.scopus-author-id24464208500
person.identifier.scopus-author-id6508057930
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf25bda81-63c2-417a-ba3f-72433472ac36
relation.isAuthorOfPublicationa5a8faa7-12a5-4b1c-bced-44c895677397
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscoveryf25bda81-63c2-417a-ba3f-72433472ac36

Files

Original bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
ART_Ana Rita Dias.pdf
Size:
2.42 MB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
txt.txt
Size:
1.74 KB
Format:
Plain Text
Download

Collections

ESS - CQB - Artigos
ESS - CISA - Artigos