Publication
Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress
| dc.contributor.author | Machado, Manuela D. | |
| dc.contributor.author | Soares, Eduardo V. | |
| dc.date.accessioned | 2015-01-06T14:34:27Z | |
| dc.date.available | 2015-01-06T14:34:27Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | The impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwateralga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells wereexposed to three nominal concentrations of each metal: low (closed to 72 h-EC10values), intermediate(closed to 72 h-EC50values) and high (upper than 72 h-EC90values). The exposure to low metal concen-trations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations anincrease of cell volume was observed; this effect was particularly notorious for Cd and less pronouncedfor Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations ofmetals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an oppositeeffect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases inP. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus;and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrationsresulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after thesecond nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). Thedifferent impact of metals on algal cell volume and cell-cycle progression, suggests that different toxic-ity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining andcell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of thepollutants, in P. subcapitata, and help in the elucidation of their different modes of action. | por |
| dc.identifier.doi | 10.1016/j.aquatox.2013.11.017 | |
| dc.identifier.issn | 0166-445X | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/5308 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.ispartofseries | Aquatic Toxicology;Vol. 147 | |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0166445X13003329 | por |
| dc.subject | Algal growth | por |
| dc.subject | Biovolume | por |
| dc.subject | Cell division | por |
| dc.subject | Cell proliferation | por |
| dc.subject | Cell size | por |
| dc.subject | Metal toxicity | por |
| dc.title | Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 6 | por |
| oaire.citation.startPage | 1 | por |
| oaire.citation.title | Aquatic Toxicology | por |
| oaire.citation.volume | 147 | por |
| person.familyName | Soares | |
| person.givenName | Eduardo | |
| person.identifier | 93637 | |
| person.identifier.ciencia-id | D612-0D0E-4984 | |
| person.identifier.orcid | 0000-0003-2280-5291 | |
| person.identifier.rid | O-5908-2015 | |
| person.identifier.scopus-author-id | 7102060173 | |
| rcaap.rights | closedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | b3ebdee1-84fa-4183-a1d8-c3cdcaaef219 | |
| relation.isAuthorOfPublication.latestForDiscovery | b3ebdee1-84fa-4183-a1d8-c3cdcaaef219 |