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Neurogenic inflammation in allergen-challenged obese mice: a missing link in the obesity-asthma association?

2012-06-26Journal article Restricted access
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dc.contributor.authorRamalho, Renata
dc.contributor.authorAlmeida, Joana
dc.contributor.authorBeltrão, Marília
dc.contributor.authorPirraco, Ana
dc.contributor.authorCosta, Raquel
dc.contributor.authorSokhatska, Oksana
dc.contributor.authorGuardão, Luísa
dc.contributor.authorPalmares, Carmo
dc.contributor.authorGuimarães, João Tiago
dc.contributor.authorDelgado, Luís
dc.contributor.authorMoreira, André
dc.contributor.authorSoares, Raquel
dc.date.accessioned2023-06-12T10:35:37Z
dc.date.available2023-06-12T10:35:37Z
dc.date.issued2012-06-26
dc.description.abstractA number of studies have shown an association between obesity and asthma. Controversy remains on the mechanisms supporting this association. In this study we aimed to assess neurogenic inflammation in a model of diet-induced obesity and allergen-challenged mice. High fat diet-induced (HFD) obese Balb/c mice were sensitized and challenged with ovalbumin (OVA). Glucose, insulin, OVA-specific IgE and substance P (SP), and the main tachykinin involved in neurogenic inflammation, were quantified in sera. Cell counts were performed in bronchoalveolar lavage fluid (BALF). The extent of peribronchial infiltrates was estimated on lung tissue sections and inflammation was score based on inflammatory cell counts surrounding the bronchi. Obesity per se and allergen-sensitization per se increased serum SP (P = .027, P = .004, respectively). Further increased was observed in obese-sensitized mice (P = .007). Obese-sensitized mice also showed higher insulin (P = .0016), OVA-specific IgE (P = .016), peribronchial inflammatory score (P = .045), and tendency for higher glycemia. The interaction of obesity and asthma on SP levels was confirmed (P = .005, R2 = 0.710). SP was positively correlated with metabolic (glycemia, r = 0.539, P = .007) and allergic inflammation parameters (BALF eosinophils, r = 0.445, P = 0.033; BALF mast cells, r = 0.574, P = .004; peribronchial inflammation score, r = 0.661, P < .001; and OVA-specific IgE, r = 0.714, P < .001). Our findings provide support to the neurogenic inflammation link between obesity and asthma in mice. These two conditions independently increased SP and the presence of both pathologies further increased this level. Neurogenic inflammation may be a previously unrecognized mechanism beyond the obese-asthma phenotype. Further studies are need to confirm this role of SP in human obesity-asthma association.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRenata Ramalho, Joana Almeida, Marília Beltrão, Ana Pirraco, Raquel Costa, Oksana Sokhatska, Luísa Guardão, Carmo Palmares, João Tiago Guimarães, Luís Delgado, André Moreira & Raquel Soares (2012) Neurogenic inflammation in allergen-challenged obese mice: a missing link in the obesity-asthma association?, Experimental Lung Research, 38:6, 316-324, DOI: 10.3109/01902148.2012.699589pt_PT
dc.identifier.doi10.3109/01902148.2012.699589pt_PT
dc.identifier.eissn1521-0499
dc.identifier.issn0190-2148
dc.identifier.urihttp://hdl.handle.net/10400.22/23119
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.3109/01902148.2012.699589pt_PT
dc.subjectAsthmapt_PT
dc.subjectNeurogenic inflammationpt_PT
dc.subjectObesitypt_PT
dc.subjectSpecific IgEpt_PT
dc.subjectSubstance Ppt_PT
dc.titleNeurogenic inflammation in allergen-challenged obese mice: a missing link in the obesity-asthma association?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage324pt_PT
oaire.citation.startPage316pt_PT
oaire.citation.titleExperimental Lung Researchpt_PT
oaire.citation.volume38 (6)pt_PT
person.familyNameALMEIDA
person.givenNameJOANA
person.identifier.ciencia-id1317-637D-8EE0
person.identifier.orcid0000-0001-8043-9053
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5ec466aa-7e58-47b6-a45b-b9ee3e6f6552
relation.isAuthorOfPublication.latestForDiscovery5ec466aa-7e58-47b6-a45b-b9ee3e6f6552

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