Publication
Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7–10)
| dc.contributor.author | Amaral, I. F. | |
| dc.contributor.author | Neiva, I. | |
| dc.contributor.author | Silva, F. Ferreira da | |
| dc.contributor.author | Sousa, Susana R. | |
| dc.contributor.author | Piloto, A. M. | |
| dc.contributor.author | Lopes, C. D. F. | |
| dc.contributor.author | Barbosa, Mário A. | |
| dc.contributor.author | Kirkpatrick, C. J. | |
| dc.contributor.author | Pêgo, A. P. | |
| dc.date.accessioned | 2014-01-17T12:52:56Z | |
| dc.date.available | 2014-01-17T12:52:56Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | The present study aimed to develop a pre-endothelialized chitosan (CH) porous hollowed scaffold for application in spinal cord regenerative therapies. CH conduits with different degrees of acetylation (DA; 4% and 15%) were prepared, characterized (microstructure, porosity and water uptake) and functionalized with a recombinant fragment of human fibronectin (rhFNIII7–10). Immobilized rhFNIII7–10 was characterized in terms of amount (125I-radiolabelling), exposure of cell-binding domains (immunofluorescence) and ability to mediate endothelial cell (EC) adhesion and cytoskeletal rearrangement. Functionalized conduits revealed a linear increase in immobilized rhFNIII7–10 with rhFNIII7–10 concentration, and, for the same concentration, higher amounts of rhFNIII7–10 on DA 4% compared with DA 15%. Moreover, rhFNIII7–10 concentrations as low as 5 and 20 lgml 1 in the coupling reaction were shown to provide DA 4% and 15% scaffolds, respectively, with levels of exposed cell-binding domains exceeding those observed on the control (DA 4% scaffolds incubated in a 20 lgml 1 human fibronectin solution). These grafting conditions proved to be effective in mediating EC adhesion/cytoskeletal organization on CH with DA 4% and 15%, without affecting the endothelial angiogenic potential. rhFNIII7–10 grafting to CH could be a strategy of particular interest in tissue engineering applications requiring the use of endothelialized porous matrices with tunable degradation rates. | por |
| dc.identifier.doi | 10.1016/j.actbio.2012.10.029 | pt_PT |
| dc.identifier.issn | 1742-7061/$ | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/3343 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.ispartofseries | Acta Biomaterialia; Vol. 9, Issue 3 | |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S174270611200517X | por |
| dc.subject | Three-dimensional scaffolds | por |
| dc.subject | Surface grafting | por |
| dc.subject | Protein radiolabelling | por |
| dc.subject | Protein conformation | por |
| dc.subject | Spinal cord injury | por |
| dc.title | Endothelialization of chitosan porous conduits via immobilization of a recombinant fibronectin fragment (rhFNIII7–10) | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 5652 | por |
| oaire.citation.startPage | 5643 | por |
| oaire.citation.title | Acta Biomaterialia | por |
| oaire.citation.volume | 9 | por |
| person.familyName | R. Sousa | |
| person.givenName | Susana | |
| person.identifier | 820351 | |
| person.identifier.ciencia-id | 2D13-F15F-D519 | |
| person.identifier.orcid | 0000-0001-8018-4310 | |
| person.identifier.rid | D-5356-2013 | |
| person.identifier.scopus-author-id | 10045389000 | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | 13159acc-5117-4c06-9ee1-02820b9b6218 | |
| relation.isAuthorOfPublication.latestForDiscovery | 13159acc-5117-4c06-9ee1-02820b9b6218 |