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Antimicrobial, Antibiofilm, and Antioxidant Properties of Boletus edulis and Neoboletus luridiformis Against Multidrug-Resistant ESKAPE Pathogens

2022-02-24Journal article Open access
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dc.contributor.authorGarcia, Juliana
dc.contributor.authorRodrigues, Francisca
dc.contributor.authorCastro, Flávia
dc.contributor.authorAires, Alfredo
dc.contributor.authorMarques, Guilhermina
dc.contributor.authorSaavedra, Maria José
dc.date.accessioned2023-01-27T10:39:32Z
dc.date.available2023-01-27T10:39:32Z
dc.date.issued2022-02-24
dc.description.abstractMultidrug-resistant ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) has become the most recurrent global cause of skin and soft-tissue infections, belonging to the WHO priority pathogens list. Successful therapy remains challenging and entails the assessment of novel and successful antibiotics. In this study, mushrooms are considered a valuable and unique source of natural antimicrobial compounds. Therefore, this study aimed to evaluate the antimicrobial and antibiofilm properties of Boletus edulis (B. edulis) and Neoboletus luridiformis (N. luridiformis) aqueous and methanolic extracts against ESKAPE isolates from clinical wound infections. Disk diffusion and microdilution methods were used to assess the antimicrobial activity. Phytochemical characterization was achieved by analysis of total phenols, orthodiphenols content, and antioxidant activity as well as by high-performance liquid chromatography-diode array detector (HPLC-DAD). Human foreskin fibroblasts-1 (HFF-1) cell viability was performed by the MTT assay. Aqueous and methanolic extracts of B. edulis and N. luridiformis showed antimicrobial and antibiofilm properties against multidrug-resistant bacteria, although with different efficacy rates. The results showed that there is a convincing relation between the content of phenolic compounds, antioxidant activity, and antimicrobial activity suggesting that the presence of phenolic compounds may explain the biological effects. HPLC analysis revealed high levels of protocatechuic acid, homogentisic acid, pyrogallol, gallic acid, p-catechin, and dihydroxybenzoic acid in the aqueous extract of B. edulis, explaining the highest antimicrobial and antibiofilm properties. Importantly, the mushrooms extracts were non-cytotoxic at all the tested concentrations. Overall, the tested mushrooms extracts are good candidates to further explore its use in the prevention of wound infection, particularly by multidrug-resistant pathogenspt_PT
dc.description.sponsorshipThis study was funded by the project I&T Companies in Co-promotion FungiTech, Norte-01-0247-FEDER-033788; National Funds by FCT—Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020 (CITAB-Center for the Research and Technology of Agro-Environmental and Biological Sciences). JG wish to acknowledge the project AquaValor—Centro de Valorização e Transferência de Tecnologia da Água (NORTE-01-0246-FEDER-000053), supported by Norte Portugal Regional Operational Programme (NORTE, 2020). FR was thankful for her contract (CEECIND/01886/2020) financed by FCT/MCTES—CEEC Individual 2020 Program Contractpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fnut.2021.773346pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21934
dc.language.isoengpt_PT
dc.publisherFrontiers Mediapt_PT
dc.relationNorte-01-0247-FEDER-033788pt_PT
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationCEECIND/01886/2020pt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fnut.2021.773346/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntibiotic resistancept_PT
dc.subjectWound infectionpt_PT
dc.subjectESKAPE bacteriapt_PT
dc.subjectWild mushroompt_PT
dc.subjectAntibiofilmpt_PT
dc.titleAntimicrobial, Antibiofilm, and Antioxidant Properties of Boletus edulis and Neoboletus luridiformis Against Multidrug-Resistant ESKAPE Pathogenspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
oaire.citation.titleFrontiers in Nutritionpt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNamePinto Lisboa Martins Rodrigues Sarmento
person.givenNameFrancisca
person.identifier.ciencia-id4F19-3D98-2CBA
person.identifier.orcid0000-0001-8803-0041
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication176ed0e9-7da4-4265-bc19-50b694ea58ab
relation.isAuthorOfPublication.latestForDiscovery176ed0e9-7da4-4265-bc19-50b694ea58ab
relation.isProjectOfPublication8ba52ad3-ac0f-4921-8293-939db56f1c68
relation.isProjectOfPublication.latestForDiscovery8ba52ad3-ac0f-4921-8293-939db56f1c68

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