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Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro

2020Journal article Open access
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dc.contributor.authorLameirão, Fátima
dc.contributor.authorPinto, Diana
dc.contributor.authorF. Vieira, Elsa
dc.contributor.authorF. Peixoto, Andreia
dc.contributor.authorFreire, Cristina
dc.contributor.authorSut, Stefania
dc.contributor.authorDall’Acqua, Stefano
dc.contributor.authorCosta, Paulo
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorRodrigues, Francisca
dc.date.accessioned2022-01-13T15:30:12Z
dc.date.available2022-01-13T15:30:12Z
dc.date.issued2020
dc.description.abstractChestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4–46 min) and temperature (34–76 °C) in the antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total phenolic compounds (TPC) of chestnut shells extracts. The optimal extraction conditions were obtained at 70 °C for 40 min. The optimal extract was characterized regarding phenolic profile, radical scavenging capacity, and effects on intestinal and dermal cell lines. The optimal extract revealed high amounts of ellagic acid (40.4 µg/mg dw), followed by caffeic acid derivative (15.4 µg/mg dw) and epigallocatechin (15.3 µg/mg dw). Indeed, the extract exhibited the highest scavenging efficiencies against NO● (IC50 = 0.1 µg/mL) and HOCl (IC50 = 0.7 µg/mL) and did not conducted to a decrease on HaCaT and HFF-1 viability up to 100 μg/mL. Oppositely, a decrease on Caco-2 and HT29-MTX viability was observed. This study suggests that UAE could be a sustainable option to valorize chestnut shells as raw material for different industries.pt_PT
dc.description.sponsorshipThe authors are grateful to Sortegel for the samples. This work received financial support from project PTDC/ASP-AGR/29277/2017—Castanea sativa shells as a new source of active ingredients for Functional Food and Cosmetic applications: A sustainable approach, supported by national funds by FCT/MCTES and co-supported by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE 2020—Programa Operacional Competitividade e Internacionalização (POCI-01-0145-FEDER-029277). The work was also supported by UID/QUI/50006/2020 with funding from FCT/MCTES through national funds and by the Applied Molecular Biosciences Unit-UCIBIO, which is financed by national funds from FCT/MCTES (UID/Multi/04378/2020). Acknowledgments Diana Pinto is thankful for the PhD grant (SFRH/BD/144534/2019) financed by POPH-QREN and subsidized by the European Science Foundation and Ministério da Ciência, Tecnologia e Ensino Superior. Elsa F. Vieira and Andreia Peixoto acknowledge the FCT for their work contract (CEECIND/03988/2018 and Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19, respectively), supported by national funds (OE).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/antiox9030267pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/19459
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationTDC/ASP-AGR/29277/2017pt_PT
dc.relationPOCI-01-0145-FEDER-029277pt_PT
dc.relationUID/QUI/50006/2020pt_PT
dc.relationUID/Multi/04378/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/9/3/267/htmpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectCastanea sativa shellspt_PT
dc.subjectUltrasound-assisted extraction (UAE)pt_PT
dc.subjectResponse surface Methodologypt_PT
dc.subjectPhenolicspt_PT
dc.titleGreen-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitropt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue3pt_PT
oaire.citation.startPage267pt_PT
oaire.citation.titleAntioxidantspt_PT
oaire.citation.volume9pt_PT
person.familyNamePinto
person.familyNameelsa
person.familyNameDelerue-Matos
person.familyNamePinto Lisboa Martins Rodrigues Sarmento
person.givenNameDiana
person.givenNamevieira
person.givenNameCristina
person.givenNameFrancisca
person.identifier.ciencia-id591C-826B-9BF4
person.identifier.ciencia-idD41C-E7B7-92F1
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-id4F19-3D98-2CBA
person.identifier.orcid0000-0001-8534-3739
person.identifier.orcid0000-0003-0301-6182
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0001-8803-0041
person.identifier.ridO-6135-2016
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id56527841500
person.identifier.scopus-author-id24167217900
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9732a79c-4ed0-43a9-a7f5-1229806f44bf
relation.isAuthorOfPublication33d008c0-bcff-4d0e-8386-a41af9166bb7
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication176ed0e9-7da4-4265-bc19-50b694ea58ab
relation.isAuthorOfPublication.latestForDiscovery9732a79c-4ed0-43a9-a7f5-1229806f44bf

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