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The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies

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dc.contributor.authorPaiva, Sandra
dc.contributor.authorStrachotová, Dita
dc.contributor.authorKučerová, Helena
dc.contributor.authorHlaváček, Otakar
dc.contributor.authorMota, Sandra
dc.contributor.authorCasal, Margarida
dc.contributor.authorPalková, Zdena
dc.contributor.authorVáchová, Libuše
dc.date.accessioned2019-07-29T16:11:17Z
dc.date.available2019-07-29T16:11:17Z
dc.date.issued2013
dc.description.abstractOn solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a 'saturable' component of the transport, which requires the presence of a functional Jen1p transporter, and a 'non-saturable' component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1042/BJ20120312pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14498
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPortland Presspt_PT
dc.relation.publisherversionhttp://www.biochemj.org/content/454/3/551.longpt_PT
dc.subjectAmmoniapt_PT
dc.subjectBiological Transport, Activept_PT
dc.subjectCarboxylic Acidspt_PT
dc.subjectDiffusionpt_PT
dc.subjectGene Knockout Techniquespt_PT
dc.subjectLactic Acidpt_PT
dc.subjectMonocarboxylic Acid Transporterspt_PT
dc.subjectRepressor Proteinspt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectSaccharomyces cerevisiae Proteinspt_PT
dc.subjectSignal Transductionpt_PT
dc.subjectSymporterspt_PT
dc.titleThe transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast coloniespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage558pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage551pt_PT
oaire.citation.titleBiochemical Journalpt_PT
oaire.citation.volume454pt_PT
person.familyNameMota
person.givenNameSandra
person.identifier.ciencia-id0A19-207B-6C63
person.identifier.orcid0000-0002-2803-7230
person.identifier.scopus-author-id6603695681
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf73a5c8e-1621-435f-a34f-c3cc83924875
relation.isAuthorOfPublication.latestForDiscoveryf73a5c8e-1621-435f-a34f-c3cc83924875

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