Publication
Exploring peracetic acid and acidic pH tolerance of antibiotic-resistant non-typhoidal Salmonella and Enterococcus faecium from diverse epidemiological and genetic backgrounds
| dc.contributor.author | Rebelo, Andreia | |
| dc.contributor.author | Duarte, Bárbara | |
| dc.contributor.author | Freitas, Ana R. | |
| dc.contributor.author | Peixe, Luísa | |
| dc.contributor.author | Antunes, Patrícia | |
| dc.contributor.author | Novais, Carla | |
| dc.date.accessioned | 2024-12-16T15:30:31Z | |
| dc.date.available | 2024-12-16T15:30:31Z | |
| dc.date.issued | 2023-09-16 | |
| dc.description.abstract | Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals’ stomach) or organic acids (e.g., feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains of specific serotypes, clonal lineages, or sources in human/animal pathogens: namely, non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This study evaluated the acidic pH (Mueller–Hinton acidified with HCl) and peracetic acid (PAA) susceptibility of Efm (n = 72) and NTS (n = 60) from diverse epidemiological/genetic backgrounds and with multiple antibiotic resistance profiles. Efm minimum growth/survival pH was 4.5–5.0/3.0–4.0, and for NTS it was 4.0–4.5/3.5–4.0. Efm distribution among acidic pH values showed that only isolates of clade-non-A1 (non-hospital associated) or the food chain were more tolerant to acidic pH compared to clade-A1 (hospital-associated clones) or clinical isolates (p < 0.05). In the case of NTS, multidrug-resistant (MDR) isolates survived better in acidic pH (p < 0.05). The PAA MIC/MBC for Efm was 70–120/80–150 mg/L, and for NTS, it was 50–70/60–100 mg/L. The distribution of Efm among PAA concentrations showed that clade-A1 or MDR strains exhibited higher tolerance than clade-non-A1 or non-MDR ones (p < 0.05). NTS distribution also showed higher tolerance to PAA among non-MDR and clinical isolates than food chain ones (p < 0.05) but there were no differences among different serogroups. This unique study identifies specific NTS or Efm populations more tolerant to acidic pH or PAA, emphasizing the need for further research to tailor controlled measures of public health and food safety within a One Health framework. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Rebelo, A., Duarte, B., Freitas, A. R., Peixe, L., Antunes, P., & Novais, C. (2023). Exploring peracetic acid and acidic pH tolerance of antibiotic-resistant non-typhoidal Salmonella and Enterococcus faecium from diverse epidemiological and genetic backgrounds. Microorganisms, 11(9), Artigo 9. https://doi.org/10.3390/microorganisms11092330 | pt_PT |
| dc.identifier.doi | 10.3390/microorganisms11092330 | pt_PT |
| dc.identifier.eissn | 2076-2607 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/26868 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | This work was financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences—UCIBIO, the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB, by the AgriFood XXI I&D&I project (NORTE-01-0145-FEDER-000041) co-financed by the European Regional Development Fund (ERDF) and through the NORTE 2020 (Programa Operacional Regional do Norte 2014/2020), and by the exploratory project EXPL/SAU-INF/0261/2021. Andreia Rebelo was supported by a PhD fellowship from FCT (SFRH/BD/137100/2018), co-financed by European Social Fund through Norte Portugal Regional Operational Programme (NORTE 2020). ARF acknowledges the Junior Research Position (CEECIND/02268/2017) granted by FCT—Fundação para a Ciência e a Tecnologia. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. | pt_PT |
| dc.relation.publisherversion | https://www.mdpi.com/2076-2607/11/9/2330 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Biocides | pt_PT |
| dc.subject | One health | pt_PT |
| dc.subject | Food chain | pt_PT |
| dc.subject | Antimicrobial resistance | pt_PT |
| dc.title | Exploring peracetic acid and acidic pH tolerance of antibiotic-resistant non-typhoidal Salmonella and Enterococcus faecium from diverse epidemiological and genetic backgrounds | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Microorganisms | pt_PT |
| oaire.citation.volume | 11(9) | pt_PT |
| person.familyName | Rebelo | |
| person.givenName | Andreia | |
| person.identifier.ciencia-id | A81C-7A59-ECAD | |
| person.identifier.orcid | 0000-0003-2218-6005 | |
| person.identifier.rid | T-9024-2017 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isAuthorOfPublication | 913dc5cd-9e92-456d-88f5-419571a87f85 | |
| relation.isAuthorOfPublication.latestForDiscovery | 913dc5cd-9e92-456d-88f5-419571a87f85 |