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An Innovative Approach for Tailoring Molecularly Imprinted Polymers for Biosensors—Application to Cancer Antigen 15-3

2024Journal article Open access
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datacite.subject.fosEngenharia e Tecnologia
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorOliveira, Daniela dos Santos
dc.contributor.authorOliveira, Andreia Sofia Rodrigues
dc.contributor.authorMendonça, Patrícia Vitorino
dc.contributor.authorCoelho, Jorge Fernando Jordão
dc.contributor.authorMoreira, Felismina Teixeira Coelho
dc.contributor.authorSales, Maria Goreti Ferreira
dc.contributor.authorMoreira, Felismina
dc.contributor.authorDOS SANTOS OLIVEIRA, DANIELA
dc.date.accessioned2025-02-11T12:11:41Z
dc.date.available2025-02-11T12:11:41Z
dc.date.issued2024
dc.description.abstractThis work presents a novel approach for tailoring molecularly imprinted polymers (MIPs) with a preliminary stage of atom transfer radical polymerization (ATRP), for a more precise definition of the imprinted cavity. A well-defined copolymer of acrylamide and N,N′-methylenebisacrylamide (PAAm-co-PMBAm) was synthesized by ATRP and applied to gold electrodes with the template, followed by a crosslinking reaction. The template was removed from the polymer matrix by enzymatic/chemical action. The surface modifications were monitored via electrochemical impedance spectroscopy (EIS), having the MIP polymer as a non-conducting film designed with affinity sites for CA15-3. The resulting biosensor exhibited a linear response to CA15-3 log concentrations from 0.001 to 100 U/mL in PBS or in diluted fetal bovine serum (1000×) in PBS. Compared to the polyacrylamide (PAAm) MIP from conventional free-radical polymerization, the ATRP-based MIP extended the biosensor’s dynamic linear range 10-fold, improving low concentration detection, and enhanced the signal reproducibility across units. The biosensor demonstrated good sensitivity and selectivity. Overall, the work described confirmed that the process of radical polymerization to build an MIP material influences the detection capacity for the target substance and the reproducibility among different biosensor units. Extending this approach to other cancer biomarkers, the methodology presented could open doors to a new generation of MIP-based biosensors for point-of-care disease diagnosis.eng
dc.description.sponsorshipThis research was funded by the Fundação para a Ciência e Tecnologia via the OligoFIT project (JPCOFUND2/0001/2019), FCT/CAPES cooperation, and PhD Grant (to DO, reference SFRH/BD/137832/2018).
dc.identifier.citationdos Santos Oliveira, D.; Oliveira, A.S.R.; Mendonça, P.V.; Coelho, J.F.J.; Moreira, F.T.C.; Sales, M.G.F. An Innovative Approach for Tailoring Molecularly Imprinted Polymers for Biosensors—Application to Cancer Antigen 15-3. Biosensors 2024, 14, 222. https://doi.org/ 10.3390/bios14050222
dc.identifier.doi10.3390/bios14050222
dc.identifier.issn2079-6374
dc.identifier.urihttp://hdl.handle.net/10400.22/29456
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relation.hasversionhttps://www.mdpi.com/2079-6374/14/5/222
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectelectrochemical biosensors
dc.subjectatom transfer radical polymerization
dc.subjectcancer biomarkers
dc.subjectprotein-imprinted polymers
dc.subjectgold screen-printed electrodes
dc.subjectcancer antigen 15-3
dc.titleAn Innovative Approach for Tailoring Molecularly Imprinted Polymers for Biosensors—Application to Cancer Antigen 15-3eng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.titleBiosensors
oaire.citation.volume14
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMoreira
person.familyNameDOS SANTOS OLIVEIRA
person.givenNameFelismina
person.givenNameDANIELA
person.identifier1589429
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idD319-DEC9-9B9F
person.identifier.orcid0000-0001-7159-2638
person.identifier.orcid0000-0003-2763-9501
person.identifier.orcid0000-0003-4755-5903
person.identifier.orcid0000-0001-9351-1704
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9936-7336
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-7159-2638
person.identifier.scopus-author-id23486193000
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication1b256c49-05f4-4958-9a13-8cc1b795412b
relation.isAuthorOfPublication.latestForDiscovery9c13153d-a73a-49ff-b7f6-c9733bc6725e

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