Repository logo
 
Publication

Diversity of metal and antibiotic resistance genes in Enterococcus spp. from the last century reflects multiple pollution and genetic exchange among phyla from overlapping ecosystems

2021Journal article Restricted access
Simple item page
dc.contributor.authorRebelo, Andreia
dc.contributor.authorMourão, Joana
dc.contributor.authorFreitas, Ana R.
dc.contributor.authorDuarte, Bárbara
dc.contributor.authorSilveira, Eduarda
dc.contributor.authorSanchez-Valenzuela, António
dc.contributor.authorAlmeida, Agostinho
dc.contributor.authorBaqueroik, Fernando
dc.contributor.authorCoqueik, Teresa M.
dc.contributor.authorPeixe, Luísa
dc.contributor.authorAntunes, Patrícia
dc.contributor.authorNovais, Carla
dc.date.accessioned2021-07-23T10:25:34Z
dc.date.available2021-07-23T10:25:34Z
dc.date.issued2021
dc.description.abstractArsenic (As), mercury (Hg), and copper (Cu) are among the major historical and contemporary metal pollutants linked to global anthropogenic activities. Enterococcus have been considered indicators of fecal pollution and antibiotic resistance for years, but its largely underexplored metallome precludes understanding their role as metal pollution bioindicators as well. Our goal was to determine the occurrence, diversity, and phenotypes associated with known acquired genes/operons conferring tolerance to As, Hg or Cu among Enterococcus and to identify their genetic context (381 field isolates from diverse epidemiological and genetic backgrounds; 3547 enterococcal genomes available in databases representing a time span during 1900–2019). Genes conferring tolerance to As (arsA), Hg (merA) or Cu (tcrB) were used as biomarkers of widespread metal tolerance operons. Different variants of metal tolerance (MeT) genes (13 arsA, 6 merA, 1 tcrB) were more commonly recovered from the food-chain (arsA, tcrB) or humans (merA), and were shared with 49 other bacterial taxa. Comparative genomics analysis revealed that MeT genes occurred in heterogeneous operons, at least since the 1900s, with an increasing accretion of antibiotic resistance genes since the 1960's, reflecting diverse antimicrobial pollution. Multiple MeT genes were co-located on the chromosome or conjugative plasmids flanked by elements with high potential for recombination, often along with antibiotic resistance genes. Phenotypic analysis of some isolates carrying MeT genes revealed up to 128× fold increase in the minimum inhibitory concentrations to metals. The main distribution of functional MeT genes among Enterococcus faecium and Enterococcus faecalis from different sources, time spans, and clonal lineages, and their ability to acquire diverse genes from multiple taxa bacterial communities places these species as good candidates to be used as model organisms in future projects aiming at the identification and quantification of bioindicators of metal polluted environments by anthropogenic activities.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRebelo, A., Mourão, J., Freitas, A. R., Duarte, B., Silveira, E., Sanchez-Valenzuela, A., Almeida, A., Baquero, F., Coque, T. M., Peixe, L., Antunes, P., & Novais, C. (2021). Diversity of metal and antibiotic resistance genes in Enterococcus spp. from the last century reflects multiple pollution and genetic exchange among phyla from overlapping ecosystems. Science of The Total Environment, 787, 147548. https://doi.org/https://doi.org/10.1016/j.scitotenv.2021.147548pt_PT
dc.identifier.doi10.1016/j.scitotenv.2021.147548pt_PT
dc.identifier.issn1879-1026
dc.identifier.urihttp://hdl.handle.net/10400.22/18163
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S004896972102619X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectFirmicutespt_PT
dc.subjectCopperpt_PT
dc.subjectArsenicpt_PT
dc.subjectMercurypt_PT
dc.subjectPlasmidspt_PT
dc.subjectOne.healthpt_PT
dc.titleDiversity of metal and antibiotic resistance genes in Enterococcus spp. from the last century reflects multiple pollution and genetic exchange among phyla from overlapping ecosystemspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage13pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleScience of The Total Environmentpt_PT
oaire.citation.volume787pt_PT
person.familyNameRebelo
person.givenNameAndreia
person.identifier.ciencia-idA81C-7A59-ECAD
person.identifier.orcid0000-0003-2218-6005
person.identifier.ridT-9024-2017
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication913dc5cd-9e92-456d-88f5-419571a87f85
relation.isAuthorOfPublication.latestForDiscovery913dc5cd-9e92-456d-88f5-419571a87f85

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_Andreia Rebelo 1.pdf
Size:
2.9 MB
Format:
Adobe Portable Document Format
Download

Collections

ESS - SA - Artigos