Publication
Molecular characterization of quinolone resistance mechanisms and extended-spectrum ß-lactamase production in Escherichia coli isolated from dogs
| dc.contributor.author | Meireles, D. | |
| dc.contributor.author | Leite-Martins, L. | |
| dc.contributor.author | Bessa, J. | |
| dc.contributor.author | Cunha, S. | |
| dc.contributor.author | Fernandes, Ruben | |
| dc.contributor.author | Matos, A. de | |
| dc.contributor.author | Manaia, C.M. | |
| dc.contributor.author | Costa, P. Martins da | |
| dc.contributor.author | Fernandes, Rúben | |
| dc.date.accessioned | 2025-05-20T08:31:06Z | |
| dc.date.available | 2025-05-20T08:31:06Z | |
| dc.date.issued | 2015-08 | |
| dc.description.abstract | The increasing prevalence of antimicrobial resistances is now a worldwide problem. Investigating the mechanisms by which pets harboring resistant strains may receive and/or transfer resistance determinants is essential to better understanding how owners and pets can interact safely. Here, we characterized the genetic determinants conferring resistance to β-lactams and quinolones in 38 multidrug-resistant Escherichia coli isolated from fecal samples of dogs, through PCR and sequencing. The most frequent genotype included the β-lactamase groups TEM (n = 5), and both TEM + CTX-M-1 (n = 5). Within the CTX-M group, we identified the genes CTX-M-32, CTX-M-1, CTX-M-15, CTX-M-55/79, CTX-M-14 and CTX-M-2/44. Thirty isolates resistant to ciprofloxacin presented two mutations in the gyrA gene and one or two mutations in the parC gene. A mutation in gyrA (reported here for the first time), due to a transversion and transition (TCG → GTG) originating a substitution of a serine by a valine in position 83 was also detected. The plasmid-encoded quinolone resistance gene, qnrs1, was detected in three isolates. Dogs can be a reservoir of genetic determinants conferring antimicrobial resistance and thus may play an important role in the spread of antimicrobial resistance to humans and other co-habitant animals. | por |
| dc.identifier.citation | Meireles, D., Leite-Martins, L., Bessa, L. J., Cunha, S., Fernandes, R., de Matos, A., Manaia, C. M., & Martins da Costa, P. (2015). Molecular characterization of quinolone resistance mechanisms and extended-spectrum β-lactamase production in Escherichia coli isolated from dogs. Comparative Immunology, Microbiology and Infectious Diseases, 41, 43–48. https://doi.org/10.1016/j.cimid.2015.04.004 | |
| dc.identifier.doi | 10.1016/j.cimid.2015.04.004 | |
| dc.identifier.eissn | 1878-1667 | |
| dc.identifier.issn | 0147-9571 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/30093 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation | PEst-OE/EQB/LA0016/2013 and PTDC/AAC-AMB/113840/2009 | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S0147957115000296?via%3Dihub | |
| dc.rights.uri | N/A | |
| dc.subject | Dogs | |
| dc.subject | Escherichia coli | |
| dc.subject | Antimicrobial resistance | |
| dc.subject | Quinolones | |
| dc.title | Molecular characterization of quinolone resistance mechanisms and extended-spectrum ß-lactamase production in Escherichia coli isolated from dogs | por |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 48 | |
| oaire.citation.startPage | 43 | |
| oaire.citation.title | Comparative Immunology, Microbiology and Infectious Diseases | |
| oaire.citation.volume | 41 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Fernandes | |
| person.givenName | Rúben | |
| person.identifier | 635792 | |
| person.identifier.ciencia-id | 0D1F-4090-E82A | |
| person.identifier.orcid | 0000-0001-8933-3984 | |
| person.identifier.scopus-author-id | 57640135700 | |
| relation.isAuthorOfPublication | 917f7713-0a35-4ef0-ae56-0784f7fc2187 | |
| relation.isAuthorOfPublication.latestForDiscovery | 917f7713-0a35-4ef0-ae56-0784f7fc2187 |