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Validation of an HPLC-DAD method for quercetin quantification in nanoparticles

2023-12-17Journal article Open access
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dc.contributor.authorCarvalho, Daniel
dc.contributor.authorJesus, Ângelo
dc.contributor.authorPinho, Cláudia
dc.contributor.authorFerraz Oliveira, Rita
dc.contributor.authorMoreira, Fernando
dc.contributor.authorOliveira, Ana Isabel
dc.date.accessioned2024-02-01T10:19:40Z
dc.date.available2024-02-01T10:19:40Z
dc.date.issued2023-12-17
dc.description.abstractThe evaluation of the efficacy of incorporation of quercetin in nanoparticles is crucial, both for the development and quality control of pharmaceutical formulations. The validation of analytical methods for the precise quantification of quercetin is useful for the evaluation of various potential quercetin delivery systems and quercetin pharmacokinetics. This work aimed to validate a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for quercetin detection and quantification in nanoparticles. Different mobile phase conditions and detection wavelengths (254 and 368 nm) were tested, and the major validation parameters were assessed (precision, accuracy, linearity, sensitivity, stability, and selectivity). The best peak resolution was obtained when quercetin was analyzed at 368 nm with a mobile phase of 1.5% acetic acid and a water/acetonitrile/methanol ratio of 55:40:5. Under these conditions, quercetin also eluted rapidly (retention time of 3.6 min). The method proved to be linear (R2 > 0.995), specific, and repeatable (variation coefficient between 2.4% and 6.7%) and presented intermediate precision (variation coefficient between 7.2% and 9.4%). The accuracy of the analysis ranged between 88.6% and 110.7%, and detection and quantification limits were 0.046 and 0.14 µg/mL, respectively. Quercetin solutions were more stable when stored at 4 °C than at room temperature or −20 °C. This validated method satisfied more parameters of bias assessment than most recent methods for quercetin determination and presented itself as more sensitive and efficient than general spectrophotometric methods. The method was successfully used for the analysis of quercetin incorporation in nanoparticles and will be evaluated in the future for its adequacy for the determination of quercetin in more complex matrices.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarvalho, D., Jesus, Â., Pinho, C., Oliveira, R. F., Moreira, F., & Oliveira, A. I. (2023). Validation of an HPLC-DAD method for quercetin quantification in nanoparticles. Pharmaceuticals, 16(12), Artigo 12. https://doi.org/10.3390/ph16121736pt_PT
dc.identifier.doi10.3390/ph16121736pt_PT
dc.identifier.eissn1424-8247
dc.identifier.urihttp://hdl.handle.net/10400.22/24922
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1424-8247/16/12/1736pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnalysispt_PT
dc.subjectChromatographypt_PT
dc.subjectHPLCpt_PT
dc.subjectQuercetinpt_PT
dc.subjectValidationpt_PT
dc.titleValidation of an HPLC-DAD method for quercetin quantification in nanoparticlespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12pt_PT
oaire.citation.startPage1736pt_PT
oaire.citation.titlePharmaceuticalspt_PT
oaire.citation.volume16pt_PT
person.familyNameJesus
person.familyNameFerraz Oliveira
person.familyNameMoreira
person.familyNameOliveira
person.givenNameÂngelo
person.givenNameRita
person.givenNameFernando
person.givenNameAna Isabel
person.identifier2947760
person.identifier.ciencia-idA91F-3585-4F42
person.identifier.ciencia-idFD1F-2E0C-1254
person.identifier.ciencia-id5F16-AB92-94D0
person.identifier.ciencia-id9212-F501-E9EF
person.identifier.orcid0000-0003-3972-8253
person.identifier.orcid0000-0003-3933-5740
person.identifier.orcid0000-0002-3452-1459
person.identifier.orcid0000-0003-4420-6476
person.identifier.ridA-4404-2012
person.identifier.scopus-author-id56153841300
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1cbff3da-9800-4bd7-8d84-fd8d9ca819b2
relation.isAuthorOfPublication6c1b7213-1303-47eb-bfa7-ecbef6a9b8d9
relation.isAuthorOfPublication81213e80-09a5-4655-93dc-e54199edb2fe
relation.isAuthorOfPublication4b3fe293-2740-469f-97d3-99d0b107b5e0
relation.isAuthorOfPublication.latestForDiscovery4b3fe293-2740-469f-97d3-99d0b107b5e0

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