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Competitive electrochemical immunosensor for the detection of unfolded p53 protein in blood as biomarker for Alzheimer’s disease

2019Journal article Open access
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dc.contributor.authorAmor-Gutiérrez, Olaya
dc.contributor.authorCosta Rama, Estefanía
dc.contributor.authorArce-Varas, Noemi
dc.contributor.authorMartínez-Rodríguez, Carmen
dc.contributor.authorNovelli, Antonello
dc.contributor.authorFernández-Sánchez, María Teresa
dc.contributor.authorCosta-García, Agustín
dc.date.accessioned2021-03-11T16:46:51Z
dc.date.available2021-03-11T16:46:51Z
dc.date.issued2019
dc.description.abstractAlzheimer's disease is one of the most common causes of dementia nowadays, and its prevalence increases over time. Because of this and the difficulty of its diagnosis, accurate methods for the analysis of specific biomarkers for an early diagnosis of this disease are much needed. Recently, the levels of unfolded isoform of the multifunctional protein p53 in plasma have been proved to increase selectively in Alzheimer's Disease patients in comparison with healthy subjects, thus entering the list of biomarkers that can be used for the diagnosis of this illness. We present here the development of an electrochemical immunosensor based on nanostructured screen-printed carbon electrodes for the quantification of unfolded p53 in plasma samples. The sensor shows a suitable linear range (from 2 to 50 nM) for its application in real blood samples and a very low limit of detection (0.05 nM). The concentration of unfolded p53 has been accurately detected in plasma of elderly people in healthy conditions, subjects with mild cognitive impairment (MCI) and Alzheimer's Disease (AD) subjects, obtaining results with no significant differences to those provided by an ELISA assay. These results support the possibility of measuring unfolded p53 levels with a cheap, simple and miniaturized device with a promising future for point-of-care applications in the early diagnosis of Alzheimer's dementia.pt_PT
dc.description.sponsorshipThis work has been supported by the FC-15-GRUPIN14-021 project from the Asturias Regional Government and the CTQ2014-58826-R and MINECO-18-CTQ2017-86994-R projects from the Spanish Ministry of Economy and Competitiveness (MINECO). O. Amor-Gutiérrez thanks Vicerrectorado de Investigación from University of Oviedo for the award of a grant “Ayudas para la realización de tesis doctorales” (PAPI-18-PF-13) through Plan de Apoyo y Promoción de la Investigación.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aca.2019.09.042pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/17450
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0003267019311134?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAlzheimer's diseasept_PT
dc.subjectp53 proteinpt_PT
dc.subjectUnfolded p53pt_PT
dc.subjectElectrochemical immunosensorpt_PT
dc.subjectScreen-printed electrodept_PT
dc.subjectGold nanoparticlespt_PT
dc.titleCompetitive electrochemical immunosensor for the detection of unfolded p53 protein in blood as biomarker for Alzheimer’s diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleAnalytica Chimica Actapt_PT
person.familyNameCosta Rama
person.givenNameEstefanía
person.identifier.ciencia-id3614-E403-0136
person.identifier.orcid0000-0002-5357-9269
person.identifier.ridJ-5815-2018
person.identifier.scopus-author-id36703129800
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc5426470-28f7-4ec9-98d5-58910259931c
relation.isAuthorOfPublication.latestForDiscoveryc5426470-28f7-4ec9-98d5-58910259931c

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