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Advisor(s)
Abstract(s)
Methicillin-resistant Staphylococcus aureus (MRSA) is a highly pathogenic multiple-drug resistant (MDR) microorganism that is most prevalent in the community. It has been found that MRSA strains can also contain genes that encode the panton valentine leukocidin toxin (PVL) and several genes that confer resistance to a various number of antimicrobial agents. The aim of this study was to determine the presence of MRSA (mecA gene) and PVL toxin genes (lukS-PV and lukF-PV) in strains isolated from two hospitals. The susceptibility to a various number of antimicrobial agents was tested and its clonality also studied. The results indicated a frequency of approximately 50% of MRSA and a frequency of 9% of isolates carrying the lukFS-PVL gene. Regarding the susceptibility patterns of isolates under study it was concluded that for most antimicrobial agents used MRSA strains reveal to be resistant to more antimicrobials than the isolates that lack mecA gene. Regarding fingerprinting method it was possible to observe a quite fixed number of strains among pathogen causing hospitalacquiring infection. The explanation for these findings is complex and multi-factorial. The high diversity of clones suggests diverse genetic backgrounds rather than the global spread of a single clone.
Description
Keywords
MRSA PVL MDR mPCR RAPD
Pedagogical Context
Citation
Silva, N., Prudêncio, C., Tomaz, C., & Fernandes, R. (2011). Antimicrobial susceptibility in clinical isolates of Staphylococcus aureus harbouring of mecA and lukFS-PV genes in Northern Portugal. Em Science and Technology Against Microbial Pathogens (pp. 275–280). WORLD SCIENTIFIC. https://doi.org/10.1142/9789814354868_0053
Publisher
World Scientific