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Advisor(s)
Abstract(s)
A validação metodológica permite evidenciar se os resultados obtidos por um método são confiáveis, tornando-se um elemento essencial na qualidade.
Neste estudo, cujo objectivo é validar a metodologia enzimática para determinação de oxalato na urina, incluíram-se 30 amostras de urina de 24horas, pré-tratadas e estudadas em dois auto-analisadores.
Os valores dos limites de detecção e quantificação foram 0,98 e 0,79 mg/L, respectivamente. Para a exactidão obteve-se p=0,713. Na precisão intermédia entre corridas não existem diferenças estatisticamente significativas, em oposição às repetições. Para a linearidade obteve-se r=1 e a sensibilidade foi 0,6%.
Conclui-se que o método se mostrou exacto e linear.
Method validation gives evidence if the results obtained by a method are reliable, making it an essential element in quality. In this study, whose aim is to validate the enzymatic method for determination of oxalate in urine, were included 30 urine samples of 24 hours, pre-treated and studied in two auto-analyzers. The values of limit of detection and limit of quantification were 0.98 and 0.79 mg / L, respectively. For the trueness was obtained p=0,713. In the intermediate precision, it appears that between runs there aren´t differences statistically significant, as opposed to repetitions. For the linearity was obtained r=1 and a sensitivity of 0.6%. We concluded that the method was exact and linear.
Method validation gives evidence if the results obtained by a method are reliable, making it an essential element in quality. In this study, whose aim is to validate the enzymatic method for determination of oxalate in urine, were included 30 urine samples of 24 hours, pre-treated and studied in two auto-analyzers. The values of limit of detection and limit of quantification were 0.98 and 0.79 mg / L, respectively. For the trueness was obtained p=0,713. In the intermediate precision, it appears that between runs there aren´t differences statistically significant, as opposed to repetitions. For the linearity was obtained r=1 and a sensitivity of 0.6%. We concluded that the method was exact and linear.
Description
Keywords
Validação Parâmetros de validação Oxalato urinário Validation Validation parameters Urinary oxalate
Citation
Grande, C., Farinha, R., Figueiredo, I., Moreira, A., Neves, F., & Sousa, M. M. A. de S. e. (2010). Validação de metodologia analítica para determinação de oxalato na urina. I Congresso Internacional da Saúde Gaia-Porto. https://recipp.ipp.pt/handle/10400.22/1300
Publisher
Instituto Politécnico do Porto. Escola Superior de Tecnologia da Saúde do Porto - Politema