Repository logo
 
Publication

Uncovering the microglia response during neonatal Group B Streptococcus meningitis

2023-05Conference object Open access
Simple item page
dc.contributor.authorSoares, Joana
dc.contributor.authorLorga, Inês
dc.contributor.authorBravo, Joana
dc.contributor.authorSummavielle, Teresa
dc.contributor.authorNova, Manuel Vila
dc.contributor.authorBonifácio Andrade, Elva
dc.date.accessioned2024-05-23T13:41:56Z
dc.date.available2024-05-23T13:41:56Z
dc.date.issued2023-05
dc.description.abstractGroup B Streptococcus (GBS) remains the most common bacterial cause of meningitis in neonates. Microglia, the brain resident immune cells, have a critical role in the development of neural circuits. However, the role of GBS infection on microglia activation and neurological sequelae remains poorly characterised. Here, we aimed to evaluate whether GBS induces changes in microglia profile during the acute phase of infection, using a mouse model that mimics key steps of GBS pathophysiology in humans. Female C57BL/6 mice were intra-vaginally inoculated with GBS during gestation, and CFU analysis was performed on postnatal days (P) 1, 3 and 5. Bacterial colonisation was found at all ages, peaking at P3. When analysing the status of microglia by flow cytometry in the whole brain of male pups at P3, an overall activation was observed in the infected group. Mainly, we found a significant increase in microglia frequency, as well as the mean fluorescence intensities (MFIs) of CD45, CD11b and F4/80. Additionally, we also analysed some microglial receptors that are important neuro-immune regulators with relevant functions during development. We observed increased CX3CR1 expression in microglia, whereas Sirp and CD200r were not altered. Moreover, analysing the cortex and hippocampus, relevant regions for cognition, we found similar numbers in Iba1+ cells, a known microglia marker, in the hippocampus of infected pups. In contrast, a significant decrease was observed in the cortex, suggesting altered migration of these cells. Furthermore, microglia phagocytosis was increased in the cortex of infected pups but not in the hippocampus. Interestingly, quantification of neurons revealed a significant decrease in the hippocampus of infected pups while being increased in the cortex, compared with age-match controls. Altogether, our results show that GBS meningitis alters the neonatal microglia profile. Further studies will be necessary to better understand the microglia inflammatory state after GBS infection.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSoares, J., Lorga, I., Bravo, J., Summavielle, T., Vilanova, M., & Andrade, E. B. (2023). Uncovering the microglia response during neonatal Group B Streptococcus meningitis. Livro de Resumos do 16º Encontro de Investigação Jovem da U.Porto, 922.pt_PT
dc.identifier.isbn978-989-746-356-3
dc.identifier.urihttp://hdl.handle.net/10400.22/25552
dc.language.isoengpt_PT
dc.publisherUniversidade do Portopt_PT
dc.relationThis work was supported by National Funds through Fundação para a Ciência e a Tecnologia (FCT), I.P., under the project EXPL/SAU-INF/1217/2021.pt_PT
dc.relation.publisherversionhttps://www.up.pt/ijup/wp-content/uploads/sites/892/2023/06/Livro-de-Resumos_final.pdfpt_PT
dc.subjectGroup B Streptococcuspt_PT
dc.subjectNeonatal meningitispt_PT
dc.subjectMicrogliapt_PT
dc.titleUncovering the microglia response during neonatal Group B Streptococcus meningitispt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
oaire.citation.issue16ªpt_PT
oaire.citation.startPage922pt_PT
oaire.citation.titleLivro de Resumos do 16.º Encontro de Investigação Jovem da U.Portopt_PT
person.familyNameSummavielle
person.familyNameBonifácio Andrade
person.givenNameTeresa
person.givenNameElva
person.identifier677706
person.identifier.ciencia-idC41E-0816-5C85
person.identifier.ciencia-id511C-11AA-7980
person.identifier.orcid0000-0003-2548-6281
person.identifier.orcid0000-0002-1941-580X
person.identifier.ridC-9776-2012
person.identifier.scopus-author-id6603092949
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication207ee2de-85a0-4144-9e7e-b376c600e065
relation.isAuthorOfPublicatione3b8883f-57b2-4b83-afa1-88e663ed8d1f
relation.isAuthorOfPublication.latestForDiscovery207ee2de-85a0-4144-9e7e-b376c600e065

Files

Collections

ESS - CF - Posters apresentados em eventos científicos
ESS - APCT - Posters apresentados em eventos científicos