Repository logo
 
Publication

Human salivary α-amylase (EC.3.2.1.1) activity and periodic acid and schiff reactive (PAS) staining: a useful tool to study polysaccharides at an undergraduate level

2006-11-03Journal article Open access
Simple item page
dc.contributor.authorFernandes, Ruben
dc.contributor.authorCorreia, Rossana
dc.contributor.authorFonte, Rosália
dc.contributor.authorPrudêncio, Cristina
dc.date.accessioned2021-09-28T08:00:31Z
dc.date.available2021-09-28T08:00:31Z
dc.date.issued2006-11-03
dc.description.abstractHealth science education is presently in discussion throughout Europe due to the Bologna Declaration. Teaching basic sciences such as biochemistry in a health sciences context, namely in allied heath education, can be a challenging task since the students of preclinical health sciences are not often convinced that basic sciences are clinically valuable (J. R. Rudland, S. C. Rennie ( 2003) The determination of the relevance of basic sciences learning objectives to clinical practice using a questionnaire survey, Med. Educ. (Oxf.) 37, 962-965; E. C. Wragg ( 2003) How can we determine the relevance of basic sciences learning objectives to clinical practice?, Med. Educ. ( Oxf.) 37, 948-949). Thus, nowadays teachers are compelled to use their imagination to be able to elaborate laboratory sessions aiming for the understanding of theoretical concepts that are also clinically related: in other words, basic concepts and skills that underlie the competencies demanded of the future health professional. In the present work, we describe a set of laboratory sessions implemented in the discipline of biochemistry, belonging to the first year of several courses of allied health professionals, which can also be implemented in other health sciences courses. These sessions focus on the characteristics and properties of carbohydrates. The exercises we propose include two different laboratory practical sessions based on a histopathological routine technique known as periodic acid and Schiff reactive that is currently used to detect sugar metabolic and tumor diseases ( J. M. T. Rivera, C. T. Lopez, B. C. Segui ( 2001) Bioquimica Estructural: Conceptos y Tests, Tebar Flores, Madrid). The methodology described enables the demonstration of some biochemical properties of polysaccharides, namely animal and vegetable, and the catalytic activity of the human salivary alpha-amylase (EC.3.2.1.1) enzyme. A further comparison between alpha-amylase activity in vitro and in situ is also possible by the proposed methodology. Additionally, to this extent, a comparison between the results of the learning improvement that occurred after the implementation of this tool is presented.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFernandes, R., Correia, R., Fonte, R., & Prudêncio, C. (2006). Human salivary α-amylase (EC.3.2.1.1) activity and periodic acid and schiff reactive (PAS) staining: A useful tool to study polysaccharides at an undergraduate level. Biochemistry and Molecular Biology Education, 34(4), 294-299. https://doi.org/https://doi.org/10.1002/bmb.2006.494034042636pt_PT
dc.identifier.doi10.1002/bmb.2006.494034042636pt_PT
dc.identifier.issn1539-3429
dc.identifier.urihttp://hdl.handle.net/10400.22/18582
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIUBMBpt_PT
dc.relation.publisherversionhttps://iubmb.onlinelibrary.wiley.com/doi/10.1002/bmb.2006.494034042636pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiochemistrypt_PT
dc.subjectCarbohydratespt_PT
dc.subjectAlpha-amylasept_PT
dc.subjectHistochemistrypt_PT
dc.titleHuman salivary α-amylase (EC.3.2.1.1) activity and periodic acid and schiff reactive (PAS) staining: a useful tool to study polysaccharides at an undergraduate levelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage299pt_PT
oaire.citation.startPage294pt_PT
oaire.citation.titleBiochemistry and Molecular Biology Educationpt_PT
oaire.citation.volume34pt_PT
person.familyNameFernandes
person.familyNamePrudêncio
person.givenNameRúben
person.givenNameCristina
person.identifier635792
person.identifier1200571
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery881a8ad5-ab13-4e49-89f4-08ca61cc81e3

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_Ruben Fernandes.pdf
Size:
838.8 KB
Format:
Adobe Portable Document Format
Download

Collections

ESS - CQB - Artigos
ESS - CISA - Artigos