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Advisor(s)
Abstract(s)
Um dos maiores desafios encontrados na cirurgia maxilofacial envolve o preenchimento de grandes defeitos, sendo necessário um biomaterial moldável, alternativo ao autoenxerto ósseo, que promova não só a formação óssea como também a integração de tecidos moles.
O nosso grupo desenvolveu um sistema injetável para regeneração óssea composto por microesferas de HAp ricas em Sr e um veículo de alginato reticulado com Sr. Os resultados obtidos, inspiraram-nos para desenhar um material injetável de bicamada, composto por uma camada inferior, promotora de regeneração óssea e uma camada superior, promotora de integração com tecidos moles. Foram também incorporados no material plasma rico em plaquetas (PRP) e membranas fetais (FMs), dado serem ricos em fatores bioativos.
A interface entre camadas manteve-se estável mesmo quando o material foi sujeito a forças de compressão. Verificou-se um aumento do número de células e da atividade metabólica, nas hMSCs e fibroblastos, quando em contacto com FMs, assim como um aumento da capacidade de mineralização das hMSCs. A análise proteómica mostrou que a incorporação de fatores bioativos resultou no enriquecimento de proteínas envolvidas na matrix celular, nos dois tipos celulares. Adicionalmente, foi observado um aumento na diferenciação osteoblástica nas formulações contendo FMs, sendo mais evidente quando associado PRP. Concluindo, este biomaterial apresenta-se promissor para reconstrução maxilofacial.
A current big challenge in maxillofacial field involves large defects filling, where a moldable biomaterial, alternative to bone autografts, is required, ideally promoting both bone formation and soft-tissue integration. Our group has previously developed an injectable system for bone regeneration composed of Sr-rich HAp microspheres, delivered in an lginate vehicle crosslinked in situ with Sr. The excellent results obtained inspired us to design a bilayer injectable biomaterial, composed of a bone-like layer (BL), to induce bone regeneration, and a gingival-like layer (GL) for periodontal tissue integration. The incorporation of platelet-rich plasma (PRP) and fetal menbranes (FMs), as natural biocompounds reservoirs, was also implemented. The bilayer biomaterial presented a stable interface between layers, even when subjected to compressive forces. Both cell types (hMSCs and fibroblasts) exhibit an increase in number and metabolic activity, in response to FMs. Furthermore, hMSCs increasd its mineralization capacity in both formulations containing FMs. Proteomic analysis revealed that incorporation of bioactive factors resulted in an ECM-related protein enrichment in both hGFs and in the promotion os hMSCs osteogenic defferentiation. This is particularly evident whn assocating FMs and PRP. The results obtained indicate that the innovative developed bilayer material provides a very promising multifunctional approach for oral and maxilofacial reconstruction.
A current big challenge in maxillofacial field involves large defects filling, where a moldable biomaterial, alternative to bone autografts, is required, ideally promoting both bone formation and soft-tissue integration. Our group has previously developed an injectable system for bone regeneration composed of Sr-rich HAp microspheres, delivered in an lginate vehicle crosslinked in situ with Sr. The excellent results obtained inspired us to design a bilayer injectable biomaterial, composed of a bone-like layer (BL), to induce bone regeneration, and a gingival-like layer (GL) for periodontal tissue integration. The incorporation of platelet-rich plasma (PRP) and fetal menbranes (FMs), as natural biocompounds reservoirs, was also implemented. The bilayer biomaterial presented a stable interface between layers, even when subjected to compressive forces. Both cell types (hMSCs and fibroblasts) exhibit an increase in number and metabolic activity, in response to FMs. Furthermore, hMSCs increasd its mineralization capacity in both formulations containing FMs. Proteomic analysis revealed that incorporation of bioactive factors resulted in an ECM-related protein enrichment in both hGFs and in the promotion os hMSCs osteogenic defferentiation. This is particularly evident whn assocating FMs and PRP. The results obtained indicate that the innovative developed bilayer material provides a very promising multifunctional approach for oral and maxilofacial reconstruction.
Description
Keywords
Biomaterial Proteómica Regeneração de tecido periodental Bicamada Biomaterials Proteomics Periodontal tissue regeneration Bilayer