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Advisor(s)
Abstract(s)
Celiac disease (CD) is a gluten-induced autoimmune enteropathy characterized by the presence of
antibodies against gliadin (AGA) and anti-tissue transglutaminase (anti-tTG) antibodies. A disposable
electrochemical dual immunosensor for the simultaneous detection of IgA and IgG type AGA and antitTG
antibodies in real patient’s samples is presented. The proposed immunosensor is based on a dual
screen-printed carbon electrode, with two working electrodes, nanostructured with a carbon–metal
hybrid system that worked as the transducer surface. The immunosensing strategy consisted of the
immobilization of gliadin and tTG (i.e. CD specific antigens) on the nanostructured electrode surface. The
electrochemical detection of the human antibodies present in the assayed serum samples was carried out
through the antigen–antibody interaction and recorded using alkaline phosphatase labelled anti-human
antibodies and a mixture of 3-indoxyl phosphate with silver ions was used as the substrate. The analytical
signal was based on the anodic redissolution of enzymatically generated silver by cyclic voltammetry.
The results obtained were corroborated with commercial ELISA kits indicating that the developed sensor
can be a good alternative to the traditional methods allowing a decentralization of the analyses towards
a point-of-care strategy.
Description
Keywords
Celiac disease Tissue transglutaminase Gliadin Electrochemical immunosensors Nanomaterials Multiplexed detection