Publicação
Towards Cerebrospinal Fluid-Free Ultrasensitive Alzheimer's Diagnostics using Molecularly Imprinted Polymers
| datacite.subject.fos | Engenharia e Tecnologia | |
| datacite.subject.sdg | 09:Indústria, Inovação e Infraestruturas | |
| dc.contributor.author | Norman, B. | |
| dc.contributor.author | Arjum, A. M. | |
| dc.contributor.author | Vulpe, G. | |
| dc.contributor.author | Liu, Guoyi | |
| dc.contributor.author | Deshpande, S. | |
| dc.contributor.author | Moreira, F. | |
| dc.date.accessioned | 2025-02-14T18:58:09Z | |
| dc.date.available | 2025-02-14T18:58:09Z | |
| dc.date.issued | 2024-07-28 | |
| dc.description.abstract | Neurofibrillary tangles (NFTs), vital in Alzheimer's disease (AD) diagnosis, consist of Tau-441, a Tau protein with 441 residues. Originating in the entorhinal cortex, these tangles spread as AD progresses. Combining the identification of tau proteins in plasma by Zetterberg and co-workers and the shared ectodermal origin of the brain and skin, suggests the presence of AD biomarkers in ISF and blood. This study presents the development of a molecularly imprinted polymer (MIP)-based sensor surface for sensitive Tau-441 detection from ISF. The biosensor surface was created by electropolymerizing aniline in the presence of the Tau-441 template using cyclic voltammetry. A non-molecularly imprinted polymer (NIP) was developed for comparison, using phosphate-buffered saline (PBS) solution at pH 7.4. Sensor performance studies of the developed MIP sensor involved detecting Tau-441 in PBS and ISF. The MIP showed a linear decrease in current with increasing concentration of Tau-441, indicating successful binding of Tau-441 to the polymeric backbone of the MIP. The sensor was able to detect Tau-441 in the range of 50 fg/mL to 500 ng/mL (1.22 fM/L to 12.2 nM/L) achieving a limit of detection (LOD) of 587 pg/mL (12.2 fM/L) in ISF. Future investigations will explore the use of MIP-modified electrodes and microneedle patches for Tau protein detection from blood and ISF, respectively. | eng |
| dc.description.sponsorship | The authors acknowledge support from MRC AMED for the grant titled “Multi-analyte prognostic and diagnostic screening in blood and skin for Alzheimer's disease.” | |
| dc.identifier.citation | B. Norman et al., "Towards Cerebrospinal Fluid-Free Ultrasensitive Alzheimer's Diagnostics using Molecularly Imprinted Polymers,"2024 IEEE BioSensors Conference (BioSensors), Cambridge, United Kingdom, 2024, pp. 1-4, doi: 10.1109/BioSensors61405.2024.10712719 | |
| dc.identifier.doi | 10.1109/BioSensors61405.2024.10712719 | |
| dc.identifier.isbn | 979-8-3503-9513-6 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/29535 | |
| dc.language.iso | eng | |
| dc.peerreviewed | n/a | |
| dc.publisher | IEEE | |
| dc.relation.hasversion | https://ieeexplore.ieee.org/document/10712719 | |
| dc.rights.uri | N/A | |
| dc.subject | Tau-411 | |
| dc.subject | Interstitial Fluid | |
| dc.subject | molecularly imprinted polymer | |
| dc.title | Towards Cerebrospinal Fluid-Free Ultrasensitive Alzheimer's Diagnostics using Molecularly Imprinted Polymers | eng |
| dc.type | conference paper | |
| dspace.entity.type | Publication | |
| oaire.citation.conferenceDate | 2024-07-28 | |
| oaire.citation.conferencePlace | Cambridge, United Kingdom | |
| oaire.citation.title | 2024 IEEE BioSensors Conference (BioSensors) | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |