Towards Cerebrospinal Fluid-Free Ultrasensitive Alzheimer's Diagnostics using Molecularly Imprinted Polymers

Norman, B.; Arjum, A. M.; Vulpe, G.; Liu, Guoyi; Deshpande, S.; Moreira, F.

http://hdl.handle.net/10400.22/29535

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Abstract(s)

Neurofibrillary tangles (NFTs), vital in Alzheimer's disease (AD) diagnosis, consist of Tau-441, a Tau protein with 441 residues. Originating in the entorhinal cortex, these tangles spread as AD progresses. Combining the identification of tau proteins in plasma by Zetterberg and co-workers and the shared ectodermal origin of the brain and skin, suggests the presence of AD biomarkers in ISF and blood. This study presents the development of a molecularly imprinted polymer (MIP)-based sensor surface for sensitive Tau-441 detection from ISF. The biosensor surface was created by electropolymerizing aniline in the presence of the Tau-441 template using cyclic voltammetry. A non-molecularly imprinted polymer (NIP) was developed for comparison, using phosphate-buffered saline (PBS) solution at pH 7.4. Sensor performance studies of the developed MIP sensor involved detecting Tau-441 in PBS and ISF. The MIP showed a linear decrease in current with increasing concentration of Tau-441, indicating successful binding of Tau-441 to the polymeric backbone of the MIP. The sensor was able to detect Tau-441 in the range of 50 fg/mL to 500 ng/mL (1.22 fM/L to 12.2 nM/L) achieving a limit of detection (LOD) of 587 pg/mL (12.2 fM/L) in ISF. Future investigations will explore the use of MIP-modified electrodes and microneedle patches for Tau protein detection from blood and ISF, respectively.

Keywords

Tau-411 Interstitial Fluid molecularly imprinted polymer

URI

http://hdl.handle.net/10400.22/29535

Citation

B. Norman et al., "Towards Cerebrospinal Fluid-Free Ultrasensitive Alzheimer's Diagnostics using Molecularly Imprinted Polymers,"2024 IEEE BioSensors Conference (BioSensors), Cambridge, United Kingdom, 2024, pp. 1-4, doi: 10.1109/BioSensors61405.2024.10712719