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Biosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantification

2013Journal article Open access
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dc.contributor.authorOliveira, Thiago M. B. F.
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorMorais, Simone
dc.contributor.authorLima-Neto, Pedro de
dc.contributor.authorCorreia, Adriana N.
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2014-01-17T12:54:48Z
dc.date.available2014-01-17T12:54:48Z
dc.date.issued2013
dc.description.abstractThis study focused on the development of a sensitive enzymatic biosensor for the determination of pirimicarb pesticide based on the immobilization of laccase on composite carbon paste electrodes. Multi- walled carbon nanotubes(MWCNTs)paste electrode modified by dispersion of laccase(3%,w/w) within the optimum composite matrix(60:40%,w/w,MWCNTs and paraffin binder)showed the best performance, with excellent electron transfer kinetic and catalytic effects related to the redox process of the substrate4- aminophenol. No metal or anti-interference membrane was added. Based on the inhibition of laccase activity, pirimicarb can be determined in the range 9.90 ×10- 7 to 1.15 ×10- 5 molL 1 using 4- aminophenol as substrate at the optimum pH of 5.0, with acceptable repeatability and reproducibility (relative standard deviations lower than 5%).The limit of detection obtained was 1.8 × 10-7 molL 1 (0.04 mgkg 1 on a fresh weight vegetable basis).The high activity and catalytic properties of the laccase- based biosensor are retained during ca. one month. The optimized electroanalytical protocol coupled to the QuEChERS methodology were applied to tomato and lettuce samples spiked at three levels; recoveries ranging from 91.0±0.1% to 101.0 ± 0.3% were attained. No significant effects in the pirimicarb electro- analysis were observed by the presence of pro-vitamin A, vitamins B1 and C,and glucose in the vegetable extracts. The proposed biosensor- based pesticide residue methodology fulfills all requisites to be used in implementation of food safety programs.por
dc.identifier.doi10.1016/j.talanta.2012.12.017pt_PT
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10400.22/3344
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesTalanta; Vol. 106
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S003991401201051Xpor
dc.subjectEnzymatic biosensorpor
dc.subjectMulti-walled carbon nanotubespor
dc.subjectLaccasepor
dc.subjectPrimicarbpor
dc.subjectQuEChERSpor
dc.subjectVegetable cropspor
dc.titleBiosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantificationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage143por
oaire.citation.startPage137por
oaire.citation.titleTalanta
oaire.citation.volumeVol. 106por
person.familyNamede Sá Barroso
person.familyNameMorais
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameSimone
person.givenNameCristina
person.identifier1598822
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0001-6433-5801
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id7007053747
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9

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