Repository logo
 
Publication

Detection of alexandrium minutum dinoflagellate in environmental samples using electrochem

2021-08-17Journal article Open access
Simple item page
dc.contributor.authorMorais, Stephanie L.
dc.contributor.authorBarros, Piedade
dc.contributor.authorSantos, Marlene
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorGomes, Andreia C.
dc.contributor.authorBarroso, M. Fátima
dc.date.accessioned2021-11-24T16:38:07Z
dc.date.available2021-11-24T16:38:07Z
dc.date.issued2021-08-17
dc.description.abstractDinoflagellates are aquatic microorganisms that inhabit both salt and fresh waters. These microorganisms are mostly harmless, however, under certain conditions, some species rapidly re produce forming water blooms that not only discolor the waters but also compromise the health of every organism in the vicinity, as some dinoflagellates produce potent toxins deemed unsafe for human health (e.g. Alexandrium minutum). In this work, a disposable electrochemical genosensor for the detection of the toxic dinoflagellate Alexandrium minutum was developed. The analytical plat form methodology consisted in a sandwich format heterogeneous hybridization of complementary DNA sequences assay. The 70 bp A. minutum-specific targeting probe, the 45 bp fluorescein isothi ocyanate-labelled signaling DNA probe and the 25 bp thiolated-DNA-capture probe were designed, after analyzing public databases. To maximize the complementary DNA hybridization and to avoid the formation of strong secondary structures, a mixed mercaptohexanol (MCH) and self-assembled monolayer (SAM) A. minutum-specific DNA-capture probe was immobilized onto disposable screen-printed gold electrodes (SPGE). Using chronoamperometric measurements, the enzymatic amplification of the electrochemical signal was obtained with a concentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD < 5.2 %. This electrochemical genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region of denatured genomic DNA, ex tracted from toxic dinoflagellates present in the Atlantic Ocean.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMorais, S. L., Barros, P., Santos, M., Delerue-Matos, C., Gomes, A. C., & Barroso, M. F. (2017). Detection of Alexandrium minutum dinoflagellate in environ mental samples using electrochem. Chemistry proceedings.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18943
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://sciforum.net/manuscripts/10772/manuscript.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAlexandrium minutumpt_PT
dc.subjectChronoamperometrypt_PT
dc.subjectElectrochemical genosensorpt_PT
dc.subjectSandwich format hybridizationpt_PT
dc.subjectScreen-printed gold electrodespt_PT
dc.titleDetection of alexandrium minutum dinoflagellate in environmental samples using electrochempt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleChemistry proceedingspt_PT
person.familyNameGonçalves de Barros
person.familyNameSantos
person.givenNamePiedade Aurora
person.givenNameMarlene
person.identifier1508370
person.identifier.ciencia-idD210-6003-454B
person.identifier.ciencia-id8311-B967-31C4
person.identifier.orcid0000-0002-2635-251X
person.identifier.orcid0000-0001-5020-5942
person.identifier.scopus-author-id23981959400
person.identifier.scopus-author-id57110502000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa85b368a-8f13-45eb-a744-7c5810a38e2c
relation.isAuthorOfPublication8ce9ee39-a4c6-46ae-99e2-49397b550f1b
relation.isAuthorOfPublication.latestForDiscovery8ce9ee39-a4c6-46ae-99e2-49397b550f1b

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART_Piedade Barros.pdf
Size:
858.59 KB
Format:
Adobe Portable Document Format
Download

Collections

ESS - CISA - Artigos
ESS - FAR - Artigos