Publicação
Inking cell blocks improves scanner detection for diagnosis in pathology
| dc.contributor.author | Eloy, Catarina | |
| dc.contributor.author | Neves, Beatriz | |
| dc.contributor.author | Vale, João | |
| dc.contributor.author | Campelos, Sofia | |
| dc.contributor.author | Curado, Mónica | |
| dc.contributor.author | Polónia, António | |
| dc.date.accessioned | 2026-01-15T14:36:27Z | |
| dc.date.available | 2026-01-15T14:36:27Z | |
| dc.date.issued | 2023-12 | |
| dc.description.abstract | Cell blocks may be hard to be totally automatically detected by the scanner (ADS),generating incomplete whole slide images (WSIs), with areas that are not scanned,leading to possible false negative diagnosis. The aim of this study is to test if inkingthe cell blocks helps increasing ADS. Test 1: 15 cell blocks were sectioned, one halfinked black (1HB) and the other inked green (1HG). Each of the halves was individu-ally processed to generate a WSI stained by the H&E. 1HBs and 1HGs had similarscanning time (median 59 s vs. 65 s, p = .126) and file sizes (median 382 Mbvs. 381 Mb, p = .567). The black ink interfered less in the observation (2.2%vs. 44.4%; p < .001) than in the green one. Test 2: 15 cell blocks were sectioned, onehalf inked black (2HB) and the other left unstained/null (2HN). Each of the halveswas individually processed to generate three WSIs—one HE, one periodic-acid Schiff(PAS), and one immunostained by cytokeratin AE1&AE3 (CKAE1AE3). HE and PASWSIs from both 2HN and 2HB groups were all totally ADS and had similar scanningtimes and file sizes. Concerning immunostaining with CKAE1AE3: ADS (46.7%vs. 93.3%; p = .014), median time for scanning (57 s vs. 83 s; p < .001) and file size(178 Mb vs. 338 Mb; p < .001) were reduced significantly in the 2HN group in com-parison with the 2HB. Although increasing scanning time and file size, inking the cellblocks helps increasing ADS after immunostaining, improving the safety and effi-ciency of the digital pathology workflow. | eng |
| dc.identifier.citation | Eloy, C., Neves, B., Vale, J., Campelos, S., Curado, M., & Polónia, A. (2023). Inking cell blocks improves scanner detection for diagnosis in pathology. Diagnostic Cytopathology, 51(12), 779–785. https://doi.org/10.1002/dc.25224 | |
| dc.identifier.doi | 10.1002/dc.25224 | |
| dc.identifier.eissn | 1097-0339 | |
| dc.identifier.issn | 8755-1039 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/31535 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Wiley | |
| dc.relation.hasversion | https://onlinelibrary.wiley.com/doi/10.1002/dc.25224 | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Cell block | |
| dc.subject | Cytology | |
| dc.subject | Digital pathology | |
| dc.subject | Inking | |
| dc.subject | Scanning | |
| dc.title | Inking cell blocks improves scanner detection for diagnosis in pathology | eng |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 785 | |
| oaire.citation.issue | 12 | |
| oaire.citation.startPage | 779 | |
| oaire.citation.title | Diagnostic Cytopathology | |
| oaire.citation.volume | 51 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |