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Exploiting kinetic features of ORAC assay for evaluation of radical scavenging capacity

2023-02-17Journal article Open access
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dc.contributor.authorCarvalho, Joana R. B.
dc.contributor.authorMeireles, Andreia N.
dc.contributor.authorMarques, Sara S.
dc.contributor.authorGregório, Bruno J. R.
dc.contributor.authorRamos, Inês I.
dc.contributor.authorSilva, Eduarda M. P.
dc.contributor.authorBarreiros, Luisa
dc.contributor.authorSegundo, Marcela A.
dc.date.accessioned2023-04-11T16:36:11Z
dc.date.available2023-04-11T16:36:11Z
dc.date.issued2023-02-17
dc.description.abstractThe analysis and interpretation of data retrieved from Oxygen Radical Absorbance Capacity (ORAC) assays represent a challenging task. ORAC indexes originate from different mathematical approaches often lacking correct elucidation of kinetic features concerning radical scavenging reactions by antioxidant compounds. In this work, the expression of ORAC values as area under fluorescein (FL) decay curves (AUC) and lag time are critically compared. This multi-parametric analysis showed the extension of radical scavenging reactions beyond the lag time period for caffeic acid, gallic acid, reduced glutathione and quercetin, extending their antioxidant protection of FL. Ethanol delayed the reaction of both FL and antioxidant compounds with free radical species generated from 2,20 -azobis(2-amidinopropane) dihydrochloride thermolysis. Trolox equivalent values, commonly used to express ORAC values, were more affected by the differences in radical scavenging kinetics between the reference and the tested antioxidant compounds when calculated from AUC than from lag time. These findings stressed the importance of choosing calibrator compounds presenting ORAC kinetics similar to samples to prevent biased estimation of the antioxidant capacity. Additionally, the framework proposed here provides a sustainable analytical method for the evaluation of antioxidant capacity, with an AGREE score of 0.73.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarvalho, J. R. B., Meireles, A. N., Marques, S. S., Gregório, B. J. R., Ramos, I. I., Silva, E. M. P., Barreiros, L., & Segundo, M. A. (2023). Exploiting Kinetic Features of ORAC Assay for Evaluation of Radical Scavenging Capacity. Antioxidants, 12(2), 505. https://doi.org/10.3390/antiox12020505pt_PT
dc.identifier.doi10.3390/antiox12020505pt_PT
dc.identifier.eissn2076-3921
dc.identifier.urihttp://hdl.handle.net/10400.22/22712
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/12/2/505pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectORACpt_PT
dc.subjectData analysispt_PT
dc.subjectArea under curvept_PT
dc.subjectLag timept_PT
dc.subjectkineticspt_PT
dc.subjectAntioxidant capacitypt_PT
dc.titleExploiting kinetic features of ORAC assay for evaluation of radical scavenging capacitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage14pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleAntioxidantspt_PT
oaire.citation.volume12(2)pt_PT
person.familyNameBarreiros
person.givenNameLuisa
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.orcid0000-0003-3481-5809
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57

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