Repository logo
 
Publication

Sensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detection

2014-08Journal article Open access
Simple item page
dc.contributor.authorOliveira, Thiago M. B. F.
dc.contributor.authorBarroso, M. Fátima
dc.contributor.authorMorais, Simone
dc.contributor.authorAraújo, Mariana
dc.contributor.authorFreire, Cristina
dc.contributor.authorLima-Neto, Pedro de
dc.contributor.authorCorreia, Adriana N.
dc.contributor.authorOliveira, M. Beatriz P. P.
dc.contributor.authorDelerue-Matos, Cristina
dc.date.accessioned2015-01-06T14:51:19Z
dc.date.available2015-01-06T14:51:19Z
dc.date.issued2014-08
dc.description.abstractA bi-enzymatic biosensor (LACC–TYR–AuNPs–CS/GPE) for carbamates was prepared in a single step by electrodeposition of a hybrid film onto a graphene doped carbon paste electrode (GPE). Graphene and the gold nanoparticles (AuNPs) were morphologically characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, dynamic light scattering and laser Doppler velocimetry. The electrodeposited hybrid film was composed of laccase (LACC), tyrosinase (TYR) and AuNPs entrapped in a chitosan (CS) polymeric matrix. Experimental parameters, namely graphene redox state, AuNPs:CS ratio, enzymes concentration, pH and inhibition time were evaluated. LACC–TYR–AuNPs–CS/GPE exhibited an improved Michaelis–Menten kinetic constant (26.9 ± 0.5 M) when compared with LACC–AuNPs–CS/GPE (37.8 ± 0.2 M) and TYR–AuNPs–CS/GPE (52.3 ± 0.4 M). Using 4-aminophenol as substrate at pH 5.5, the device presented wide linear ranges, low detection limits (1.68×10− 9 ± 1.18×10− 10 – 2.15×10− 7 ± 3.41×10− 9 M), high accuracy, sensitivity (1.13×106 ± 8.11×104 – 2.19×108 ± 2.51×107 %inhibition M− 1), repeatability (1.2–5.8% RSD), reproducibility (3.2–6.5% RSD) and stability (ca. twenty days) to determine carbaryl, formetanate hydrochloride, propoxur and ziram in citrus fruits based on their inhibitory capacity on the polyphenoloxidases activity. Recoveries at two fortified levels ranged from 93.8 ± 0.3% (lemon) to 97.8 ± 0.3% (orange). Glucose, citric acid and ascorbic acid do not interfere significantly in the electroanalysis. The proposed electroanalytical procedure can be a promising tool for food safety control.por
dc.identifier.doi10.1016/j.bioelechem.2014.02.003
dc.identifier.urihttp://hdl.handle.net/10400.22/5312
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofseriesBioelectrochemistry;Vol. 98
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1567539414000371por
dc.subjectBi-enzymatic biosensorpor
dc.subjectGraphene modified electrodepor
dc.subjectGold nanoparticlespor
dc.subjectChitosan hybrid filmpor
dc.subjectCarbamatespor
dc.titleSensitive bi-enzymatic biosensor based on polyphenoloxidases–gold nanoparticles–chitosan hybrid film–graphene doped carbon paste electrode for carbamates detectionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage29por
oaire.citation.startPage20por
oaire.citation.titleBioelectrochemistrypor
oaire.citation.volume98por
person.familyNamede Sá Barroso
person.familyNameMorais
person.familyNameDelerue-Matos
person.givenNameMaria de Fátima
person.givenNameSimone
person.givenNameCristina
person.identifier1598822
person.identifier.ciencia-idAF16-22E5-5EBD
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0001-9011-5992
person.identifier.orcid0000-0001-6433-5801
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id25631648000
person.identifier.scopus-author-id7007053747
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication3d701c85-4580-4614-be6d-5a1abc8cf729
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_21_GRAQ_2014.pdf
Size:
1.06 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – GRAQ – Artigos