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Harvesting the power of green synthesis: gold nanoparticles tailored for prostate cancer therapy

2024-02-14Journal article Open access
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dc.contributor.authorOliveira, Marco
dc.contributor.authorSousa, André
dc.contributor.authorSá, Sara
dc.contributor.authorSoares, Sílvia
dc.contributor.authorPereira, Ana Cláudia
dc.contributor.authorRocha, Ana Catarina
dc.contributor.authorPais, Patrick
dc.contributor.authorFerreira, Diogo
dc.contributor.authorAlmeida, Cátia
dc.contributor.authorLuís, Carla
dc.contributor.authorLima, Cláudio
dc.contributor.authorAlmeida, Fábio
dc.contributor.authorGestoso, Álvaro
dc.contributor.authorDuarte, Miguel-Correa
dc.contributor.authorBarata, Pedro
dc.contributor.authorMartins-Mendes, Daniela
dc.contributor.authorBaylina, Pilar
dc.contributor.authorPereira, Carla F.
dc.contributor.authorFernandes, Rúben
dc.date.accessioned2024-04-09T15:51:41Z
dc.date.available2024-04-09T15:51:41Z
dc.date.issued2024-02-14
dc.description.abstractBiosynthetic gold nanoparticles (bAuNPs) present a promising avenue for enhancing biocompatibility and offering an economically and environmentally responsible alternative to traditional production methods, achieved through a reduction in the use of hazardous chemicals. While the potential of bAuNPs as anticancer agents has been explored, there is a limited body of research focusing on the crucial physicochemical conditions influencing bAuNP production. In this study, we aim to identify the optimal growth phase of Pseudomonas aeruginosa cultures that maximizes the redox potential and coordinates the formation of bAuNPs with increased efficiency. The investigation employs 2,6-dichlorophenolindophenol (DCIP) as a redox indicator. Simultaneously, we explore the impact of temperature, pH, and incubation duration on the biosynthesis of bAuNPs, with a specific emphasis on their potential application as antitumor agents. Characterization of the resulting bAuNPs is conducted using ATR-FT-IR, TEM, and UV-Vis spectroscopy. To gain insights into the anticancer potential of bAuNPs, an experimental model is employed, utilizing both non-neoplastic (HPEpiC) and neoplastic (PC3) epithelial cell lines. Notably, P. aeruginosa cultures at 9 h/OD600 = 1, combined with biosynthesis at pH 9.0 for 24 h at 58 ◦C, produce bAuNPs that exhibit smaller, more spherical, and less aggregated characteristics. Crucially, these nanoparticles demonstrate negligible effects on HPEpiC cells while significantly impacting PC3 cells, resulting in reduced viability, migration, and lower IL-6 levels. This research lays the groundwork for the development of more specialized, economical, and ecologically friendly treatment modalities.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOliveira, M., Sousa, A., Sá, S., Soares, S., Pereira, A. C., Rocha, A. C., Pais, P., Ferreira, D., Almeida, C., Luís, C., Lima, C., Almeida, F., Gestoso, Á., Duarte, M.-C., Barata, P., Martins-Mendes, D., Baylina, P., Pereira, C. F., & Fernandes, R. (2024). Harvesting the power of green synthesis: Gold nanoparticles tailored for prostate cancer therapy. International Journal of Molecular Sciences, 25(4), Artigo 4. https://doi.org/10.3390/ijms25042277pt_PT
dc.identifier.doi10.3390/ijms25042277pt_PT
dc.identifier.eissn1422-0067
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.22/25322
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFCT 2022.09032.PTDC; COMPETE NORTE-01-0145-FEDER-024325pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/25/4/2277pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiosynthetic gold nanoparticlespt_PT
dc.subjectP. aeruginosapt_PT
dc.subjectProstate cancerpt_PT
dc.subjectAnticancer activitypt_PT
dc.titleHarvesting the power of green synthesis: gold nanoparticles tailored for prostate cancer therapypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage23pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume25(4)pt_PT
person.familyNameFerreira
person.familyNameBAYLINA MACHADO
person.givenNameDiogo
person.givenNamePILAR
person.identifier.ciencia-id4418-B544-ACCE
person.identifier.ciencia-id1419-F23D-4920
person.identifier.orcid0000-0002-2765-0586
person.identifier.orcid0000-0002-3740-862X
person.identifier.ridB-5134-2010
person.identifier.scopus-author-id56534079700
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationf73f13d7-af7d-42d5-a828-fbe678f18d70
relation.isAuthorOfPublicationb1482a24-d9d8-419d-af68-6df1a75afb3f
relation.isAuthorOfPublication.latestForDiscoveryb1482a24-d9d8-419d-af68-6df1a75afb3f

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