Repository logo
 
Publication

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

2019-06Journal article Open access
Simple item page
dc.contributor.authorGomes, Filipa O.
dc.contributor.authorMaia, Luísa B.
dc.contributor.authorLoureiro, Joana A.
dc.contributor.authorPereira, Maria Carmo
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorMoura, Isabel
dc.contributor.authorMoura, José J.G.
dc.contributor.authorMorais, Simone
dc.date.accessioned2020-07-10T13:44:27Z
dc.date.available2020-07-10T13:44:27Z
dc.date.issued2019-06
dc.description.abstractAn enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol (DSPE-PEG)] and NOR. NOR direct electron transfer and NO bioelectrocatalysis were characterized by several electrochemical techniques. The biosensor development was also followed by scanning electron microscopy and Fourier transform infrared spectroscopy. Improved enzyme stability and electron transfer (1.96 × 10-4 cm.s-1 apparent rate constant) was obtained with the optimum SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR) ratio of 4/2.5/4 (v/v/v), which biomimicked the NOR environment. The PGE/[SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR] biosensor exhibited a low Michaelis-Menten constant (4.3 μM), wide linear range (0.44-9.09 μM), low detection limit (0.13 μM), high repeatability (4.1% RSD), reproducibility (7.0% RSD), and stability (ca. 5 weeks). Selectivity tests towards L-arginine, ascorbic acid, sodium nitrate, sodium nitrite and glucose showed that these compounds did not significantly interfere in NO biosensing (91.0 ± 9.3%-98.4 ± 5.3% recoveries). The proposed biosensor, by incorporating the benefits of biomimetic features of the phospholipid bilayer with SWCNT's inherent properties and NOR bioelectrocatalytic activity and selectivity, is a promising tool for NO.pt_PT
dc.description.sponsorshipFG thanks to Fundacão para a Ciência e a Tecnologia, MCTES (FCT/MCTES) for the fellowship grant SFRH/BD/52502/2014, which is financed by national funds and co-financed by FSE. LBM thanks to FCT/MCTES for the CEEC-Individual 2017 Program Contract. This work was supported by the PTDC/BB-BQB/0129/2014 project (FCT/MCTES), by FCT/MEC with national funds and co-funded by FEDER, and also by the Associate Laboratory Research Unit for Green Chemistry - Technologies and Processes Clean – LAQV, financed by national funds from FCT/MCTES (UID/QUI/50006/2019). The financial support from the European Union (FEDER funds through COMPETE) and National Funds (Fundação para a Ciência e Tecnologia-FCT) through project PTDC/ASP-PES/29547/2017, by FCT/MEC with national funds and co-funded by FEDER, is also acknowledged. J.A. Loureiro post-doc grant was supported by NORTE-01-0145-FEDER-000005 – LEPABE-2 ECO-INNOVATION, from North Portugal Regional Operational Program (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bioelechem.2019.01.010pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/16095
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiosensing Techniquespt_PT
dc.subjectEnzymespt_PT
dc.subjectLimit of Detectionpt_PT
dc.subjectLipid Bilayerspt_PT
dc.subjectMarinobacterpt_PT
dc.subjectNanotubespt_PT
dc.subjectNitric Oxidept_PT
dc.subjectOxidoreductasespt_PT
dc.subjectDirect electron transferpt_PT
dc.subjectEnzymatic biosensorpt_PT
dc.subjectNitric oxide reductasept_PT
dc.titleBiosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocompositept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage86pt_PT
oaire.citation.startPage76pt_PT
oaire.citation.titleBioelectrochemistrypt_PT
oaire.citation.volume127pt_PT
person.familyNameO. Gomes
person.familyNameDelerue-Matos
person.familyNameMorais
person.givenNameFilipa
person.givenNameCristina
person.givenNameSimone
person.identifier1598822
person.identifier.ciencia-id0811-1421-0FD8
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.ciencia-idB111-BBFB-F200
person.identifier.orcid0000-0002-9050-4557
person.identifier.orcid0000-0002-3924-776X
person.identifier.orcid0000-0001-6433-5801
person.identifier.ridO-4202-2015
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
person.identifier.scopus-author-id7007053747
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication99473b94-8312-4bb3-bb46-6c6c0a958be0
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication7a9c7d63-a6c7-4159-b12d-b049b8c5c8f9
relation.isAuthorOfPublication.latestForDiscovery99473b94-8312-4bb3-bb46-6c6c0a958be0

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ART_GRAQ_DELERUE_2019_14.pdf
Size:
657.6 KB
Format:
Adobe Portable Document Format
Download

Collections

ISEP – GRAQ – Artigos