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Dual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoeba

2022Journal article Open access
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dc.contributor.authorPastrana, Cristina
dc.contributor.authorGuerreiro, Joana Rafaela
dc.contributor.authorElumalai, Monisha
dc.contributor.authorCarpena-Torres, Carlos
dc.contributor.authorA, Crooke
dc.contributor.authorCarracedo Rodriguez, Juan Gonzalo
dc.contributor.authorPrado, Marta
dc.contributor.authorToral, Fernando
dc.date.accessioned2023-01-26T09:37:03Z
dc.date.available2023-01-26T09:37:03Z
dc.date.issued2022
dc.description.abstractAcanthamoebakeratitis is an aggressive and rapidly progressing ocular pathology whosemain risk factor is the use of contact lenses. An early and differential diagnosis is considered themain factor to prevent the progression and improve the prognosis of the pathology. However, currentdiagnosis techniques require time, complex and costly materials making an early diagnosis challeng-ing. Thus, there is a need for fast, accessible, and accurate methods forAcanthamoebadetection bypractitioners for timely and suitable treatment and even for contact lens user as preventive diagnosis.Here, we developed a dual-mode colorimetric-based method for fast, visual, and accurate detectionofAcanthamoebausing gold nanoparticles (AuNPs). For this strategy, AuNPs were functionalizedwith thiolated probes and the presence of targetAcanthamoebagenomic sequences, produce a colori-metric change from red to purple. This approach allows the detection of 0.02 and 0.009μM of theunamplifiedAcanthamoebagenome by the naked eye in less than 20 min and by color analysis usinga smartphone. Additionally, real samples were successfully analyzed showing the potential of thetechnology considering the lack of point-of-care tools that are mostly needed.pt_PT
dc.description.sponsorshipThis work was supported by an industrial project (ND2017/BMD7676) granted by the community of Madrid.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms232314877pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21877
dc.language.isoengpt_PT
dc.publisherMDPIpt_PT
dc.relationND2017/BMD7676pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/23/14877pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAcanthamoebapt_PT
dc.subjectAcanthamoebakeratitispt_PT
dc.subjectGold nanoparticlespt_PT
dc.subjectColorimetric assaypt_PT
dc.subjectColor coordinatespt_PT
dc.subjectOptical detectionpt_PT
dc.titleDual-Mode Gold Nanoparticle-Based Method for Early Detection of Acanthamoebapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue23pt_PT
oaire.citation.startPage14877pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
person.familyNamePastrana
person.familyNameGuerreiro
person.familyNameCarpena-Torres
person.familyNameCrooke
person.familyNameCarracedo Rodriguez
person.familyNamePrado Rodríguez
person.familyNameToral
person.givenNameCristina
person.givenNameJoana Rafaela
person.givenNameCarlos
person.givenNameAlmudena
person.givenNameJuan Gonzalo
person.givenNameMarta
person.givenNameFernando
person.identifier1781251
person.identifierK-7365-2012
person.identifier.ciencia-id6B12-98A6-18BE
person.identifier.ciencia-id0315-244A-0319
person.identifier.ciencia-idD312-F711-AE33
person.identifier.orcid0000-0003-2395-3106
person.identifier.orcid0000-0001-8719-1978
person.identifier.orcid0000-0001-9077-3557
person.identifier.orcid0000-0003-4360-1896
person.identifier.orcid0000-0003-0054-1731
person.identifier.orcid0000-0002-0529-5771
person.identifier.orcid0000-0002-7873-8916
person.identifier.ridK-5881-2015
person.identifier.scopus-author-id35730167800
person.identifier.scopus-author-id57195999980
person.identifier.scopus-author-id22936900100
person.identifier.scopus-author-id7102374834
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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